Light- and Temperature-Induced Expression of an R2R3-MYB Gene Regulates Anthocyanin Biosynthesis in Red-Fleshed Kiwifruit
Open Access
- 22 October 2019
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 20 (20), 5228
- https://doi.org/10.3390/ijms20205228
Abstract
The R2R3 MYB genes associated with the flavonoid/anthocyanidin pathway feature two repeats, and represent the most abundant classes of MYB genes in plants; however, the physiological role and regulatory function of most R2R3 MYBs remain poorly understood in kiwifruit (Actinidia). Here, genome-wide analysis identified 155 R2R3-MYBs in the ‘Red 5′ version of the Actinidia chinensis genome. Out of 36 anthocyanin-related AccR2R3-MYBs, AcMYB10 was the most highly expressed in inner pericarp of red-fleshed kiwifruit. The expression of AcMYB10 was highly correlated with anthocyanin accumulation in natural pigmentation during fruit ripening and light-/temperature-induced pigmentation in the callus. AcMYB10 is localized in the nuclei and has transcriptional activation activity. Overexpression of AcMYB10 elevates anthocyanin accumulation in transgenic A. chinensis. In comparison, A. chinensis fruit infiltrated with virus-induced gene silencing showed delayed red coloration, lower anthocyanin content, and lower expression of AcMYB10. The transient expression experiment in Nicotiana tabacum leaves and Actinidia arguta fruit indicated the interaction of AcMYB10 with AcbHLH42 might strongly activate anthocyanin biosynthesis by activating the transcription of AcLDOX and AcF3GT. In conclusion, this study provides novel molecular information about R2R3-MYBs in kiwifruit, advances our understanding of light- and temperature-induced anthocyanin accumulation, and demonstrates the important function of AcMYB10 in the biosynthesis of anthocyanin in kiwifruit.Funding Information
- National Natural Science Foundation of China (31501745, 30671433, 31171945)
This publication has 55 references indexed in Scilit:
- New insights into the regulation of anthocyanin biosynthesis in fruitsTrends in Plant Science, 2013
- An R2R3 MYB transcription factor determines red petal colour in an Actinidia (kiwifruit) hybrid populationBMC Genomics, 2013
- Genome-wide classification and expression analysis of MYB transcription factor families in rice and ArabidopsisBMC Genomics, 2012
- Genome-wide analysis of the MYB transcription factor superfamily in soybeanBMC Plant Biology, 2012
- Identification and characterisation of F3GT1 and F3GGT1, two glycosyltransferases responsible for anthocyanin biosynthesis in red‐fleshed kiwifruit (Actinidia chinensis)The Plant Journal, 2010
- The Transcription Factor VvMYB5b Contributes to the Regulation of Anthocyanin and Proanthocyanidin Biosynthesis in Developing Grape BerriesPlant Physiology, 2008
- Analysis of Transcription Factor HY5 Genomic Binding Sites Revealed Its Hierarchical Role in Light Regulation of DevelopmentTHE PLANT CELL ONLINE, 2007
- Red colouration in apple fruit is due to the activity of the MYB transcription factor, MdMYB10The Plant Journal, 2007
- Light-Induced Expression of a MYB Gene Regulates Anthocyanin Biosynthesis in Red ApplesPlant Physiology, 2006
- Two groups of MYB transcription factors share a motif which enhances trans-activation activityBiochemical and Biophysical Research Communications, 2006