The effect of supplementation of Lumbricus sp. extract in fermented foods for growth performance, body chemical composition, and hepatosomatic index of milkfish, Chanos chanos Forsskal, 1775
The quality of feed can be improved by fermenting feedstuffs and supplementing with feed additives. This study aims to determine the effect of supplementation of Lumbricus sp. extract in fermented feed on growth performance, body chemical composition, and milkfish hepatosomatic index. Milkfish with an initial weight of 17.80 ± 0.20 g head-1, rearing with a density of 20 fish per hapa net measuring 1 m3 by 24 pieces. Hapa net is installed in a pond with a water level of ± 60 cm. Research was design in completely randomized design with two factors. The first-factor was supplementation method, namely Lumbricus sp. which sprayed on feed and mixed with feed ingredients. The second factor was the dose of Lumbricus sp. extract, namely 0, 100, 200 and 300 mL kg-1 of feedstuffs. The fish was reared for 50 days and fed with 5% of fish biomass per day with feeding frequency of 3 times i.e., morning, afternoon and evening. The results showed that the supplementation Lumbricus sp. extract has no significant effect on all parameters (P>0,05). However, the level dose of Lumbricus sp. extract supplementation in feed has a significantly effect (P0,05) on survival and chemical composition of milkfish body. The best best of absolute growth (16.94±4.0 g), relative growth (48.71±5.77%), feed efficiency (40.74±10.3 %), and hepatosomatic index (1.5 ± 0.17) were found in the experiment of supplementation Lumbricus sp. extract mixed with feedstuffs at a dose of 300 mL kg1. The survival rate of milkfish was ranging from 68.33±29.3 to 91.,33±7.64%, while the body's chemical composition including protein levels, fat, ash, crude fiber, NFE / Nitrogen Free Extract, liver glycogen and muscle glycogen were 69.45±1.23 – 71.45±0.97%, 14.86±0.46 – 17.24±0.76%, 9.28±0.12 – 11.12±0.46%, 1.54±0.09 – 1.66 ± 0.13%, 1.33± 0.42 – 2.71±0.21%, 7,11±0,08 – 7,40±0,17mg g-1 and 6,13±0,44 – 6,45±0,40 mg g-1, respectively.