Structural characterization of microcrystalline and nanocrystalline cellulose from Ananas comosus L. leaves: Cytocompatibility and molecular docking studies
Open Access
- 1 January 2021
- journal article
- research article
- Published by Walter de Gruyter GmbH in Nanotechnology Reviews
- Vol. 10 (1), 793-806
- https://doi.org/10.1515/ntrev-2021-0053
Abstract
The present study focused on the preparation of microcrystalline cellulose (MCC) and nanocrystalline cellulose (NCC) from pineapple ( Ananas comosus L.) leaves using chemical treatments followed by acid hydrolysis. Pineapple leaves could be used in medical applications such as drug delivery carriers. Advanced spectroscopy techniques such as Fourier-transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) were used to analyze the physical, chemical, and morphological features of the isolated MCC and NCC; the results indicated the needle-shaped form of nanostructures with good purity and high crystallinity index of 75.00 and 76.38%, respectively. In addition, inhibition of the treated MRC-5 cells with all the samples revealed that the percentage of cell viability was less than 30%, which is an interesting finding given their role in the cytotoxicity effect of MCC and NCC. It appears that MCC and NCC derived from pineapple leaves have lower toxicity. As a result, the developed MCC and NCC can be used in pharmaceutical applications as a novel drug delivery system. Molecular docking was performed to understand the non-bonding interaction of cellulose with human acid-beta-glucosidase (β-Glc) (PDB: 1OGS). The docking result shows that cellulose unit docked within the active pocket of the enzyme by forming hydrogen bonds against ASN19, THR21, and VAL17 with distances of 2.18, 1.93, and 2.92 Å, respectively, with binding energy (−5.0 kcal/mol) resulting in close interaction of cellulose unit with the receptor.This publication has 67 references indexed in Scilit:
- Effects of hydrolysis conditions on the morphology, crystallinity, and thermal stability of cellulose nanocrystals extracted from kenaf bast fibersCellulose, 2012
- Physicochemical, spectroscopic and thermal properties of microcrystalline cellulose derived from corn cobsInternational Journal of Recycling of Organic Waste in Agriculture, 2012
- Synthesis and characterization of microcrystalline cellulose produced from bacterial celluloseJournal of Thermal Analysis and Calorimetry, 2011
- Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performanceBiotechnology for Biofuels, 2010
- AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibilityJournal of Computational Chemistry, 2009
- Coupled acid and enzyme mediated production of microcrystalline cellulose from corn cob and cotton gin wasteCellulose, 2006
- Isolation and characterization of cellulose from sugarcane bagassePolymer Degradation and Stability, 2004
- Pyrolysis behavior and kinetics of biomass derived materialsJournal of Analytical and Applied Pyrolysis, 2002
- LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions"Protein Engineering, Design and Selection", 1995
- An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-Ray DiffractometerTextile Research Journal, 1959