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A Monoclonal Antibody Specific for Pseudomonas aeruginosa Amidase

, , Alda Clemente, Paul R. Brown
Published: 1 August 2001

Abstract: Amidase from Pseudomonas aeruginosa was purified by anionic exchange chromatography and used to immunise female Balb/c mice. Monoclonal antibodies (MAbs) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. A selected IgM subclass MAb was purified from in vitro hybridoma cell line supernatant by a two-step anionic exchange chromatography. The MAb was specific for amidase from P. aeruginosa as determined by Western blotting and recognized the native and denatured forms of the enzyme.
Keywords: Cells as Fusion / Monoclonal antibody / PSEUDOMONAS AERUGINOSA / Aeruginosa Amidase / hybridoma / MAb / Amidase Amidase

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