A Monoclonal Antibody Specific for Pseudomonas aeruginosa Amidase
Published: 1 August 2001
Hybridoma , Volume 20, pp 273-279; https://doi.org/10.1089/027245701753179866
Abstract: Amidase from Pseudomonas aeruginosa was purified by anionic exchange chromatography and used to immunise female Balb/c mice. Monoclonal antibodies (MAbs) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. A selected IgM subclass MAb was purified from in vitro hybridoma cell line supernatant by a two-step anionic exchange chromatography. The MAb was specific for amidase from P. aeruginosa as determined by Western blotting and recognized the native and denatured forms of the enzyme.
Keywords: Cells as Fusion / Monoclonal antibody / PSEUDOMONAS AERUGINOSA / Aeruginosa Amidase / hybridoma / MAb / Amidase Amidase
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