A Monoclonal Antibody Specific for Pseudomonas aeruginosa Amidase
- 1 August 2001
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 20 (4), 273-279
- https://doi.org/10.1089/027245701753179866
Abstract
Amidase from Pseudomonas aeruginosa was purified by anionic exchange chromatography and used to immunise female Balb/c mice. Monoclonal antibodies (MAbs) were raised by hybridoma technology using Sp2/0 myeloma cells as fusion partner. A selected IgM subclass MAb was purified from in vitro hybridoma cell line supernatant by a two-step anionic exchange chromatography. The MAb was specific for amidase from P. aeruginosa as determined by Western blotting and recognized the native and denatured forms of the enzyme.Keywords
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