Assessment of different protocols for the isolation and purification of gut associated lymphoid cells from the gilthead seabream (Sparus aurata L.)
Open Access
- 25 November 2007
- journal article
- Published by Springer Science and Business Media LLC in Biological Procedures Online
- Vol. 9 (1), 43-55
- https://doi.org/10.1251/bpo132
Abstract
Teleost gut associated lymphoid tissue (GALT) consists of leucocyte populations located both intraepithelially and in the lamina propria with no structural organization. The present study aims to assess different protocols for the isolation of GALT cells from an important fish species in the Mediterranean aquaculture, the gilthead seabream. Mechanical, chemical and enzymatic treatments were assayed. Nylon wool columns and continuous density gradients were used for further separation of cell subpopulations. Light microscopy and flow cytometry showed that the highest density band (HD) consisted of a homogeneous lymphocytic population, whereas the intermediate density band (ID) corresponded to epithelial and secretory cells and some lymphocytes. Respiratory burst activity of total cell suspensions revealed very low numbers of potential phagocytic cells, reflecting results from light microscopy and reports in other teleost species. The present data set up the basis for future functional characterization of GALT in seabream.Keywords
This publication has 23 references indexed in Scilit:
- The influence of different management systems and age on intestinal morphology, immune cell numbers and mucin production from goblet cells in post-weaning pigsVeterinary Immunology and Immunopathology, 2006
- Intraepithelial but not lamina propria lymphocytes in the porcine gut are affected by dexamethasone treatmentVeterinary Immunology and Immunopathology, 2005
- Purification of human lamina propria plasma cells by an immunomagnetic selection methodJournal of Immunological Methods, 2004
- The immune-enhancing effects of dietary fibres and prebioticsBritish Journal of Nutrition, 2002
- The immune-enhancing effects of dietary fibres and prebioticsBritish Journal of Nutrition, 2002
- Intraepithelial and lamina propria lymphocytes show distinct patterns of apoptosis whereas both populations are active in Fas based cytotoxicity in coeliac diseaseGut, 2001
- Dietary vitamin E and rainbow trout (Oncorhynchus mykiss) phagocyte functions: effect on gut and on head kidney leucocytesFish & Shellfish Immunology, 2001
- Immunocytochemical detection of Ig-positive cells in blood, lymphoid organs and the gut associated lymphoid tissue of the turbot (Scophthalmus maximus)Fish & Shellfish Immunology, 2000
- The morphology of the immune system in teleost fishesFish & Shellfish Immunology, 1999
- Detection of MHC class II transcripts in lymphoid tissues of the common carp (Cyprinus carpio L.)Developmental & Comparative Immunology, 1995