Dynamic monitoring of vital functions and tissue re-organization in Saturnia pavonia (Lepidoptera, Saturniidae) during final metamorphosis by non-invasive MRI
Open Access
- 20 January 2022
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 12 (1), 1-13
- https://doi.org/10.1038/s41598-022-05092-3
Abstract
Magnetic resonance imaging (MRI) is the key whole-body imaging technology for observing processes within a living object providing excellent resolution and contrast between soft tissues. In the present work, we exploited the non-destructive properties of MRI to track longitudinally the dynamic changes that take place in developing pupae of the Emperor Moth (Saturnia pavonia) during the last days before eclosion. While in diapause pupae, body fluid was almost homogeneously distributed over the internal compartments, as soon as wings, legs, flight muscles and the head region were fully developed, a significant redistribution of water levels occurred between thoracic and abdominal regions. During the last two days before eclosion, the developing moths transferred substantial amounts of liquid into the gut and the labial gland, and in case of females, into developing eggs. Concomitantly, the volume of the air sacs increased drastically and their expansion/compression became clearly visible in time-resolved MR images. Furthermore, besides ventilation of the tracheal system, air sacs are likely to serve as volume reservoir for liquid transfer during development of the moths inside their pupal case. In parallel, we were able to monitor noninvasively lipid consumption, cardiac activity and haemolymph circulation during final metamorphosis.Funding Information
- Deutsche Forschungsgemeinschaft (INST 208/764-1 FUGG)
- Universitätsklinikum Düsseldorf. Anstalt öffentlichen Rechts
This publication has 41 references indexed in Scilit:
- Metamorphosis revealed: time-lapse three-dimensional imaging inside a living chrysalisJournal of The Royal Society Interface, 2013
- Non-invasive lipid measurement in living insects using NMR microscopyJournal of Experimental Biology, 2012
- Formation of a ventral cavity in lepidopteran insect pupaeApplied Entomology and Zoology, 2010
- Proximate sources of sexual size dimorphism in insects: locating constraints on larval growth schedulesEvolutionary Ecology, 2009
- Magnetic Resonance Microscopy of Flows and Compressions of the Circulatory, Respiratory, and Digestive Systems in Pupae of the Tobacco Hornworm,Manduca sextaJournal of Insect Science, 2008
- Comparison between prospective and retrospective triggering for mouse cardiac MRINMR in Biomedicine, 2006
- In vivo 3D MRI of insect brain: cerebral development during metamorphosis of Manduca sextaNeuroImage, 2005
- Herbivory in global climate change research: direct effects of rising temperature on insect herbivoresGlobal Change Biology, 2002
- Nitrogen excretion in insectsProceedings: Animal Sciences, 1988
- X-ray investigation of gas bubble formation, and water loss in tsetse fly pupaePhysiological Entomology, 1978