High genomic diversity of novel phages infecting the plant pathogen Ralstonia solanacearum, isolated in Mauritius and Reunion islands
Open Access
- 8 March 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 11 (1), 1-10
- https://doi.org/10.1038/s41598-021-84305-7
Abstract
Bacterial wilt caused by the Ralstonia solanacearum species complex (RSSC) is among the most important plant diseases worldwide, severely affecting a high number of crops and ornamental plants in tropical regions. Only a limited number of phages infecting R. solanacearum have been isolated over the years, despite the importance of this bacterium and the associated plant disease. The antibacterial effect or morphological traits of these R. solanacearum viruses have been well studied, but not their genomic features, which need deeper consideration. This study reports the full genome of 23 new phages infecting RSSC isolated from agricultural samples collected in Mauritius and Reunion islands, particularly affected by this plant bacterial pathogen and considered biodiversity hotspots in the Southwest Indian Ocean. The complete genomic information and phylogenetic classification is provided, revealing high genetic diversity between them and weak similarities with previous related phages. The results support our proposal of 13 new species and seven new genera of R. solanacearum phages. Our findings highlight the wide prevalence of phages of RSSC in infected agricultural settings and the underlying genetic diversity. Discoveries of this kind lead more insight into the diversity of phages in general and to optimizing their use as biocontrol agents of bacterial diseases of plants in agriculture.Keywords
Funding Information
- European Regional Development Fund
This publication has 41 references indexed in Scilit:
- Back to the future: evolving bacteriophages to increase their effectiveness against the pathogen Pseudomonas aeruginosa PAO1Evolutionary Applications, 2013
- Top 10 plant pathogenic bacteria in molecular plant pathologyMolecular Plant Pathology, 2012
- SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell SequencingJournal of Computational Biology, 2012
- The Filamentous Phage ϕRSS1 Enhances Virulence of Phytopathogenic Ralstonia solanacearum on TomatoPhytopathology®, 2012
- PHACTS, a computational approach to classifying the lifestyle of phagesBioinformatics, 2012
- Accelerated Profile HMM SearchesPLoS Computational Biology, 2011
- Genomes and Characterization of Phages Bcep22 and BcepIL02, Founders of a Novel Phage Type in Burkholderia cenocepaciaJournal of Bacteriology, 2011
- Detection of novel recombinases in bacteriophage genomes unveils Rad52, Rad51 and Gp2.5 remote homologsNucleic Acids Research, 2010
- Genomic Characterization of Ralstonia solanacearum Phage φRSA1 and Its Related Prophage (φRSX) in Strain GMI1000Journal of Bacteriology, 2008