Magnetic solid-phase extraction of high molecular weight peptides using stearic acid–functionalized magnetic hydroxyapatite nanocomposite: determination of some hypothalamic agents in biological samples
- 19 October 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Analytical and Bioanalytical Chemistry
- Vol. 413 (30), 7609-7623
- https://doi.org/10.1007/s00216-021-03725-6
Abstract
Therapeutic peptides have an important effect on physiological function and human health, so it is momentous to quantify and detect low levels of these biomolecules in biological samples for treatment and diagnostic purposes. In the present study, an efficient magnetic solid-phase extraction (MSPE) method was developed based on stearic acid–functionalized magnetic hydroxyapatite nanocomposite (MHAP/SA) as a novel and cost-effective adsorbent for extraction of five hypothalamic-related peptides (goserelin, octreotide, triptorelin, somatostatin, and cetrorelix) from biological samples. To characterize the morphology and physicochemical properties of MHAP/SA, Fourier transform infrared spectroscopy (FT-IR), energy-dispersive X-ray spectroscopy (EDS), field emission scanning microscopy (FE-SEM), CHNS elemental analysis, Brunauer–Emmett–Teller (BET), and vibrating sample magnetometry (VSM) were applied. Under optimum conditions, the proposed method (MSPE–HPLC–UV) represented favorable linearity with R2 ≥ 0.9987, suitable intra- and inter-day precisions (RSD ≤ 6.9% and RSD ≤ 8.1%, respectively, n = 3), and limits of detection and quantification in the range of 0.75–1.12 ng mL−1 and 2.50–3.75 ng mL−1, respectively. Eventually, the proposed method was used for the extraction and quantification of target therapeutic peptides in plasma and urine samples, and satisfactory relative recoveries were achieved in the range of 90.6–110.3%. Graphical abstractKeywords
This publication has 50 references indexed in Scilit:
- Validation of a rapid liquid chromatography–tandem mass spectrometric assay for the determination of octreotide plasma concentrationsClinical Biochemistry, 2014
- Determination of acidity constants and ionic mobilities of polyprotic peptide hormones by CZEElectrophoresis, 2013
- Doping control analysis of TB-500, a synthetic version of an active region of thymosin β4, in equine urine and plasma by liquid chromatography–mass spectrometryJournal of Chromatography A, 2012
- Quantitation of slow release triptorelin in beagle dog plasma by liquid chromatography–tandem mass spectrometryJournal of Pharmaceutical and Biomedical Analysis, 2012
- Determination of octreotide and assessment of matrix effects in human plasma using ultra high performance liquid chromatography–tandem mass spectrometryJournal of Chromatography B, 2011
- Methods for the determination of limit of detection and limit of quantitation of the analytical methodsChronicles of Young Scientists, 2011
- A thermosensitive chitosan/poly(vinyl alcohol) hydrogel containing hydroxyapatite for protein deliveryJournal of Biomedical Materials Research Part A, 2008
- Organically modified porous hydroxyapatites: A comparison between alkylphosphonate grafting and citrate chelationJournal of Solid State Chemistry, 2008
- Development and validation of a HPLC method for routine quantification of the decapeptide Cetrorelix in liposome dispersionsJournal of Pharmaceutical and Biomedical Analysis, 2004
- Radioimmunoassay of [d-Trp6]-luteinizing hormone-releasing hormone: its application to animal pharmacokinetic studies after single injection and long-acting formulation administrationRegulatory Peptides, 1986