An Investigation of Hypofrontality in an Animal Model of Schizophrenia Using Real-Time Microelectrochemical Sensors for Glucose, Oxygen, and Nitric Oxide
- 12 April 2013
- journal article
- Published by American Chemical Society (ACS) in ACS Chemical Neuroscience
- Vol. 4 (5), 825-831
- https://doi.org/10.1021/cn4000567
Abstract
Glucose, O2, and nitric oxide (NO) were monitored in real time in the prefrontal cortex of freely moving animals using microelectrochemical sensors following phencyclidine (PCP) administration. Injection of saline controls produced a decrease in glucose and increases in both O2 and NO. These changes were short-lived and typical of injection stress, lasting ca. 30 s for glucose and between 2 and 6 min for O2 and NO, respectively. Subchronic PCP (10 mg/kg) resulted in increased motor activity and increases in all three analytes lasting several hours: O2 and glucose were uncoupled with O2 increasing rapidly following injection reaching a maximum of 70% (ca. 62 μM) after ca. 15 min and then slowly returning to baseline over a period of ca. 3 h. The time course of changes in glucose and NO were similar; both signals increased gradually over the first hour post injection reaching maxima of 55% (ca. 982 μM) and 8% (ca. 31 nM), respectively, and remaining elevated to within 1 h of returning to baseline levels (after ca. 5 and 7 h, respectively). While supporting increased utilization of glucose and O2 and suggesting overcompensating supply mechanisms, this neurochemical data indicates a hyperfrontal effect following acute PCP administration which is potentially mediated by NO. It also confirms that long-term in vivo electrochemical sensors and data offer a real-time biochemical perspective of the underlying mechanisms.Keywords
This publication has 54 references indexed in Scilit:
- Hemodynamic responses in amygdala and hippocampus distinguish between aversive and neutral cues during Pavlovian fear conditioning in behaving ratsEuropean Journal of Neuroscience, 2012
- Changes in reward-related signals in the rat nucleus accumbens measured by in vivo oxygen amperometry are consistent with fMRI BOLD responses in manNeuroImage, 2012
- Brain tissue oxygen amperometry in behaving rats demonstrates functional dissociation of dorsal and ventral hippocampus during spatial processing and anxietyEuropean Journal of Neuroscience, 2010
- The role of hippocampal nitric oxide (NO) on learning and immediate, short- and long-term memory retrieval in inhibitory avoidance task in male adult ratsBehavioural Brain Research, 2009
- Modelling prefrontal cortex deficits in schizophrenia: implications for treatmentBritish Journal of Pharmacology, 2008
- NMDA Receptor Hypofunction Produces Opposite Effects on Prefrontal Cortex Interneurons and Pyramidal NeuronsJournal of Neuroscience, 2007
- Resting hypofrontality in schizophrenia: A study using near-infrared time-resolved spectroscopySchizophrenia Research, 2006
- Hypofrontality in schizophrenia: a meta‐analysis of functional imaging studiesActa Psychiatrica Scandinavica, 2004
- Cellular Bases of Brain Energy Metabolism and Their Relevance to Functional Brain Imaging: Evidence for a Prominent Role of AstrocytesCerebral Cortex, 1996
- Glucose Metabolic Rate in Normals and Schizophrenics During the Continuous Performance Test Assessed by Positron Emission TomographyThe British Journal of Psychiatry, 1990