Green Synthesized Silver Nanoparticles as Potent Antifungal Agent against Aspergillus terreus Thom
Open Access
- 26 August 2021
- journal article
- research article
- Published by Hindawi Limited in Journal of Nanomaterials
- Vol. 2021, 1-10
- https://doi.org/10.1155/2021/2992335
Abstract
Green Synthesized Silver Nanoparticles as Potent Antifungal Agent against Aspergillus terreus Thom: Medicinal plants are composed of a rich pool of biomolecules and have been increasingly recognized for their antimicrobial properties; however, increasing concerns have been put on the bioavailability features. Thus, this study is aimed at exploring the synthesis and characterization of silver nanoparticles synthesized by Chenopodium album L. leaf extract and assessing the antifungal activity against Aspergillus terreus Thom. Plant extract was prepared in methanol to synthetize silver nanoparticles, which were then characterized by Scanning Electron Microscopy (SEM), UV-Visible spectroscopy, and particle size analysis. UV-Visible analysis indicated maximum absorption at 378nm, and an average particle size was observed as 25.6nm. Oval to hexagonal shape was observed by SEM. Antifungal activity of silver nanoparticles (1, 1.5, 2, 2.5, 3, and 3.5) was addressed against A. terreus biomass. At 3.5, silver nanoparticles revealed to be highly effective, leading to 92 retardation in fungus growth. In next phase, various organic fractions, viz., chloroform, n-butanol, n-hexane, and ethyl acetate, were obtained from plant methanol extract, and the corresponding silver nanoparticles were prepared. These fractions were also assessed for antifungal activity, and n-hexane fraction led to 64 inhibition in A. terreus biomass. Following gas chromatography-mass spectrometry (GC-MS), 18 compounds were identified, namely, 1,3-cyclopentadiene-5-(1 methylethylidene and o-xylene), ethyl benzene, octadecane, nonane, decane, 2-methylheptane, n-hexadecane, 2-methylheptane, and eicosane, along with carbonyl compounds (4,4-dimethyl-3-hexanone) and phenols, like stearic acid, propionic acid hydrazide, and 2,4-di-T-butylphenol. These findings proved that C. album silver nanoparticles are highly effective against A. terreus.Keywords
Funding Information
- Fundação para a Ciência e a Tecnologia (PTDC/PSI-GER/28076/2017)
This publication has 15 references indexed in Scilit:
- Impact of Salicylic Acid and PGPR on the Drought Tolerance and Phytoremediation Potential of Helianthus annusFrontiers in Microbiology, 2018
- Aspergillus terreus Inhibits Growth and Induces Morphological Abnormalities in Pythium aphanidermatum and Suppresses Pythium-Induced Damping-Off of CucumberFrontiers in Microbiology, 2018
- Management of Ascochyta rabiei by Chenopodium album ExtractsAdvances in Weed Science, 2016
- BIOPESTICIDAL ACTIVITY OF Calotropis procera L. AGAINST Macrophomina phaseolinaAfrican Journal of Traditional, Complementary and Alternative Medicines, 2016
- Study of the Performance of the Organic Extracts ofChenopodium ambrosioidesfor Ag Nanoparticle SynthesisJournal of Nanomaterials, 2016
- Aspergillus terreus Thom a new pathogen that causes foliar blight of potatoplant pathology & quarantine, 2013
- Emerging fungal threats to animal, plant and ecosystem healthNature, 2012
- New species in Aspergillus section TerreiStudies in Mycology, 2011
- Antifungal compounds fromMelia azedarachleaves for management ofAscochyta rabiei, the cause of chickpea blightNatural Product Research, 2011
- Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extractColloids and Surfaces A: Physicochemical and Engineering Aspects, 2010