Background: Vitiligo is a common skin disease affecting 1%-2% of general population. Even though different modalities of treatment available, it remains as a difficult disease to treat. Many cases remain stable after a period of medical therapy without any further response. Surgical techniques are the only hope in such patients. The aim of this study was to evaluate the response of autologous, noncultured melanocyte keratinocyte cell transplantation in patients with stable focal vitiligo.Methods: A retrospective study of 100 cases focal vitiligo treated by autologous, noncultured melanocyte-keratinocyte cell transplantation. Serial of photographs were taken on each visit. The patents were followed for a period of 5 years. The results were analyzed on a visual analogue scale.Results: Out of total 100 patients, 44 had excellent (90 to 100%) response, 18 had good (60 to 89%) response, 12 had fair (25 to 59%) response and 26 had poor response (0 to 24%). Age and sex of the patients and size and duration of lesions, did not show significant influence on results of transplantation. Conclusions: This is a simple, safe, and effective surgical therapy for replenishing the missing melanocytes in resistant cases of vitiligo. Repigmentation lasts long with very good cosmetic acceptability.