Ozonated water antimicrobial efficacy in comparison to antimicrobial activity of different irrigant solutions against E. faecalis and E. coli

Abstract

Aim: To determine the antimicrobial efficacy of ozonated water and compare the antimicrobial activity of different irrigant solutions against E. faecalis and E. coli. Material and Method: This study was conducted in the Department of Pedodontics and Preventive Dentistry, Jaipur Dental College, Jaipur in collaboration with Dr. B. Lal Institute of Biotechnology, Jaipur to test and compare the efficacy of Ozonated Water, 3% Sodium Hypochlorite and Normal Saline in relation to Enterococcus faecalis (MTCC 439) and Escherichia coli (ATCC 25922) micro-organisms. After incubation, 21 contaminated roots were divided into 3 groups according to the irrigation regimen used i.e. Group A: Ozonated Water (7 teeth), Group B: 3% NaOCl (7 teeth) and Group C: Saline (7 teeth). All the teeth were handled with sterile gloves and sterile tweezers to prevent contamination. A sterile 5 mL syringe with 26 gauge needle was used to deliver irrigant into the canal. All experimental teeth were then flushed with distilled water to prevent potential carry-over of irrigants. Results: Results indicated that Group C showed highly significant difference from group A and Group B indicating that Group A and Group B were effective against both E. faecalis and E. coli. Results also show that there was no significant difference in efficacy of Group A and Group B. Conclusion: The results found that ozonated water has proved to be an alternative antimicrobial agent.