The investigation of bacterial adhesion of palatine tonsils epithelial cells in patient with infectious mononucleosis
Open Access
- 25 May 2021
- journal article
- Published by OU Scientific Route in EUREKA: Health Sciences
- No. 3,p. 45-52
- https://doi.org/10.21303/2504-5679.2021.001835
Abstract
Aim. To evaluate the features of pharynx tonsils mucous membrane colonization by pathogenic and opportunistic microorganisms by using the pharynx palatine tonsils epithelium electron microscopic examination of the patients with infectious mononucleosis and acute streptococcal tonsillitis. Materials and methods. Two patients – patient P., 12 years old with a confirmed diagnosis of infectious mononucleosis and patient A., 8 years old with confirmed acute streptococcal tonsillitis. The patients were taken a bacteriological examination of the mucus and epithelium scraping from the surface of the pharyngeal tonsils. Tissue samples were examined in the electron microscopy laboratory of the Lviv National University. Results and discussion. 36 tonsils epithelial tissue micro preparations of patient A. and 41 micro preparations of patient P. were studied. Streptococcus pyogenes, Str. pneumoniae, Str. viridans, Сandida albicans, as well as non-pathogenic bacteria: Diphtheroides sp., Neisseria sp., Corynebacterium spp. were identified as result of the patient P. bacteriological examination of mucus from the surface of the pharyngeal tonsils. Staph. aureus, Str. viridans, Str. pneumoniae were identified during a bacteriological examination of patient A. Eosinophils with a two-segmented nucleus, specific granularity, phagocytosed spherical bacteria in the cytoplasm were detected during the histological examination of the materials taken from the surface of the patient’s tonsils with acute tonsillitis. Research showed that bacteria were accumulated not only in the structure of extracellular detritus. Numerous bacteria accumulations were also found in the cytoplasm of the epithelial cells in the patient with infectious mononucleosis. The cell's shape looked like a bunch of grapes. Conclusions. The electron microscopic examination showed differences in the coccal flora localization: the extracellular localization of bacteria in the patient with acute bacterial tonsillitis and intraepithelial presence of the bacteria in the patient with tonsillitis during infectious mononucleosis were found.This publication has 20 references indexed in Scilit:
- Tonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar HypertrophymSystems, 2021
- Impact of Microbiota: A Paradigm for Evolving Herd Immunity against Viral DiseasesViruses, 2020
- The influence of spaceflight on the astronaut salivary microbiome and the search for a microbiome biomarker for viral reactivationMicrobiome, 2020
- Infectious mononucleosis complicated by peritonsillar abscess and postural orthostatic tachycardia syndrome: A case reportSage Open Medical Case Reports, 2020
- Condition of nasopharynx contamination in children with chronic tonsillitis and gastroesophageal reflux diseaseZaporozhye Medical Journal, 2019
- A Guide to Utilization of the Microbiology Laboratory for Diagnosis of Infectious Diseases: 2018 Update by the Infectious Diseases Society of America and the American Society for MicrobiologyaClinical Infectious Diseases, 2018
- Prevalence of High-Risk Human Papillomavirus in Tonsil Tissue in Healthy Adults and Colocalization in Biofilm of Tonsillar CryptsJAMA Otolaryngology–Head & Neck Surgery, 2018
- Age-related differences in human palatine tonsillar B cell subsets and immunoglobulin isotypesClinical and Experimental Medicine, 2015
- Bacterial penetration into tonsillar surface epithelium during infectious mononucleosis.The Journal of Laryngology & Otology, 2000
- THE USE OF LEAD CITRATE AT HIGH pH AS AN ELECTRON-OPAQUE STAIN IN ELECTRON MICROSCOPYThe Journal of cell biology, 1963