Genome sequence analysis of new plum pox virus isolates from Japan
Open Access
- 10 July 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Research Notes
- Vol. 14 (1), 1-7
- https://doi.org/10.1186/s13104-021-05683-9
Abstract
To find mutations that may have recently occurred in Plum pox virus (PPV), we collected six PPV-infected plum/peach trees from the western part of Japan and one from the eastern part. After sequencing the full-length PPV genomic RNAs, we compared the amino acid sequences with representative isolates of each PPV strain. All new isolates were found to belong to the PPV-D strain: the six isolates collected from western Japan were identified as the West-Japan strain while the one collected from eastern Japan as the East-Japan strain. Amino acid sequence analysis of these seven isolates suggested that the 1407th and 1529th amino acid residues are characteristic of the West-Japan and the East-Japan strains, respectively. Comparing them with the corresponding amino acid residues of the 47 non-Japanese PPV-D isolates revealed that these amino acid residues are undoubtedly unique. A further examination of the relevant amino acid residues of the other 210 PPV-D isolates collected in Japan generated a new hypothesis regarding the invasion route from overseas and the subsequent diffusion route within Japan: a PPV-D strain might have invaded the western part of Japan from overseas and spread throughout Japan.Keywords
Funding Information
- National Agricultural Research Organization
This publication has 8 references indexed in Scilit:
- Intra‐strain biological and epidemiological characterization of plum pox virusMolecular Plant Pathology, 2020
- MEGA X: Molecular Evolutionary Genetics Analysis across Computing PlatformsMolecular Biology and Evolution, 2018
- Analysis of Genetic Diversity of Russian Sour Cherry Plum pox virus Isolates Provides Evidence of a New StrainPlant Disease, 2018
- Plum pox virus and sharka: a model potyvirus and a major diseaseMolecular Plant Pathology, 2014
- Sharka: The Past, The Present and The FutureViruses, 2012
- Molecular Epidemiology of Plum pox virus in JapanPhytopathology®, 2011
- Prunus Host Range of Plum pox virus (PPV) in the United States by Aphid and Graft InoculationPlant Disease, 2007
- Plum pox virusand the estimated costs associated with sharka diseaseEPPO Bulletin, 2006