Purpose — To discuss the applying effectiveness analysis of magnetic resonance diffusion tensor imaging among stuttering patients. Method — Choose 81 suspected stuttering patients between May, 2018 to May, 2021 as researching objects. All of the patients did the test of magnetic resonance diffusion tensor imaging. And then the testing results was compared with clinical examination results (Gold standard), ROC curve was drawn to analyze the applying effectiveness analysis of magnetic resonance diffusion tensor imaging among stuttering patients. Results — 70 patients were diagnosed as stuttering patients from clinical examination among the 81 suspected stuttering patients. The diagnosis rate is 86.42%. After all of the patients did the test of magnetic resonance diffusion tensor imaging, 68 patients were diagnosed as stuttering patients finally. Diagnosis coincidence rate is 97.14% (P>0.05). The rFA levels of the insula, corpus callosum, and putamen on the side of stuttering lesion are lower than those on the healthy side (P0.05). The ROC curve shows that the AUC data of magnetic resonance diffusion tensor imaging in stuttering patients is 0.834, the diagnostic sensitivity is 84.31%, and the specificity is 71.48%. Conclusion — Magnetic resonance diffusion tensor imaging applying in stuttering patients can gain higher detection rate, which can provide imaging evidence for clinical diagnosis and treatment for clinical and is worthy to promoting and applying.