Correlation of plasma coagulation tests and fibrinogenClauss with rotational thromboelastometry parameters and prediction of bleeding in dogs

Abstract

Background Correlation of plasma fibrinogen concentration (fibrinogen_Clauss) with rotational thromboelastometry (ROTEM) parameters has not been investigated in dogs. Objectives To determine the correlation between plasma coagulation tests and fibrinogen_Clauss with ROTEM parameters and to evaluate their ability to predict bleeding in dogs. Animals Ninety‐seven dogs with concurrent determination of fibrinogen_Clauss and fibrin polymerization test (FIBTEM) analysis. Methods Signalment, pretreatment, clinical signs of bleeding, fibrinogen_Clauss, plasma coagulation test results, hematocrit, platelet count, FIBTEM, extrinsic (EXTEM) and intrinsic (INTEM) activated ROTEM assays were retrieved retrospectively. Correlations between fibrinogen_Clauss and FIBTEM maximum clot firmness (MCF_FIBTEM) and between prothrombin time (PT) or activated partial thromboplastin time (aPTT) and ROTEM parameters were determined. Dogs were further assigned to groups with or without clinical signs of bleeding. The prognostic significance of significantly different parameters to predict bleeding was evaluated. Results Fibrinogen_Clauss showed strong correlation with MCF_FIBTEM (r = 0.860, n = 97, P < .001). PT showed strong correlation with EXTEM clotting time (CT_EXTEM) (r = 0.839, n = 53, P < .001), and aPTT was strongly correlated with INTEM CT (CT_INTEM) (r = 0.664, n = 31, P < .001). Platelet count, PT/aPTT, EXTEM clot formation time (CFT_EXTEM), MCF_EXTEM, EXTEM maximum clot elasticity (MCE_EXTEM), and CT_INTEM were significantly different between groups. A CT_INTEM >149 seconds was 100% sensitive to detect bleeding. Conclusions and Clinical Importance The MCF_FIBTEM can be used to evaluate the effect of fibrinogen on hemostasis as an alternative to determination of fibrinogen_Clauss. In addition, CT_EXTEM and CT_INTEM are strongly correlated with PT and aPTT, respectively.