The study was conducted to examine the effect of plant growth promoting rhizobacteria; Pseudomonas punonensis LMT03 (R1), Pseudomonas plecoglossicida (R4), Pseudomonas aeruginosa DSM 50071 (R2), Alcaligenes faecalis (DBHU5) and their consortium on yield and lignin deposition of the barley crop. Consortium treated plants had the highest plant height, leaf surface area, number of fertile tillers, spike length, grains per spike, 1000 grain weight, grain yield, straw yield, total biomass, and harvest index percent and the lowest values were found in control plots. The consortium treated plant produced the highest grain yield 7976 kg/ha, while control plants produced 3200 kg/ha. In comparison to the control plant, the PGPR-treated barley plant showed dense lignin deposition in the vascular bundles of the stem section.This is the first report on the effect of P.punonensis and P. plecoglossicida on barley crop yield parameters under field conditions, also the first report on lignin deposition in barley plant treated with P. punonensis and P. plecoglossicida strains.