The carpological collection of Professor Ivo Horvat, a famous Croatian botanist of 20th century, was saved from oblivion, after its unexpected discovery at the Faculty of Veterinary Medicine in Zagreb in 2019. As many as 515 diaspores (fruits and seeds) of 486 vascular plant taxa were systematised and digitised and the nomenclature was updated. A comprehensive comparison of Horvat’s carpological and herbarium collection (ZAHO) revealed a large amount of overlap. A large photo-catalogue was created and will be publicly accessible through the Flora Croatica Database.