1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling
Open Access
- 12 April 2021
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Cell and Developmental Biology
Abstract
The increasing intensity of environmental radiofrequency electromagnetic fields (RF-EMF) has increased public concern about its health effects. Of particular concern are the influences of RF-EMF exposure on the development of the brain. The mechanisms of how RF-EMF acts on the developing brain are not fully understood. Here, based on high-throughput RNA sequencing techniques, we revealed that transcripts related to neurite development were significantly influenced by 1800 MHz RF-EMF exposure during neuronal differentiation. Exposure to RF-EMF remarkably decreased the total length of neurite and the number of branch points in neural stem cells-derived neurons and retinoic acid-induced Neuro-2A cells. The expression of Eph receptors 5 (EPHA5), which is required for neurite outgrowth, was inhibited remarkably after RF-EMF exposure. Enhancing EPHA5 signaling rescued the inhibitory effects of RF-EMF on neurite outgrowth. Besides, we identified that cAMP-response element-binding protein (CREB) and RhoA were critical downstream factors of EPHA5 signaling in mediating the inhibitory effects of RF-EMF on neurite outgrowth. Together, our finding revealed that RF-EMF exposure impaired neurite outgrowth through EPHA5 signaling. This finding explored the effects and key mechanisms of how RF-EMF exposure impaired neurite outgrowth and also provided a new clue to understanding the influences of RF-EMF on brain development.Keywords
This publication has 79 references indexed in Scilit:
- The relationship between adolescents’ well-being and their wireless phone use: a cross-sectional studyEnvironmental Health, 2013
- The Small GTPase RhoA Is Required for Proper Locomotor Circuit AssemblyPLOS ONE, 2013
- A chemical genetic approach reveals distinct EphB signaling mechanisms during brain developmentNature Neuroscience, 2012
- Ephrin regulation of synapse formation, function and plasticityMolecular and Cellular Neuroscience, 2012
- RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genomeBMC Bioinformatics, 2011
- Pathway-specific engagement of ephrinA5-EphA4/EphA5 system of the substantia nigra pars reticulata in cocaine-induced responsesProceedings of the National Academy of Sciences of the United States of America, 2011
- Ephrin-A5 and EphA5 Interaction Induces Synaptogenesis during Early Hippocampal DevelopmentPLOS ONE, 2010
- Continuous exposure to 900MHz GSM-modulated EMF alters morphological maturation of neural cellsNeuroscience Letters, 2009
- Distribution of EphA5 receptor protein in the developing and adult mouse nervous systemJournal of Comparative Neurology, 2009
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001