A Cell‐Culture Technique to Encode Glyco‐Nanoparticles Selectivity
- 7 October 2021
- journal article
- research article
- Published by Wiley in Chemistry – An Asian Journal
- Vol. 16 (23), 3900-3904
- https://doi.org/10.1002/asia.202101015
Abstract
Nanoparticles (NPs) embedded with bioactive ligands such as carbohydrates, peptides, and nucleic acid have emerged as a potential tool to target biological processes. Traditional in vitro assays performed under statistic conditions may result in non-specific outcome sometimes, mainly because of the sedimentation and self-assembly nature of NPs. Inverted cell-culture assay allows for flexible and accurate detection of the receptor-mediated uptake and cytotoxicity of NPs. By combining this technique with glyco-gold nanoparticles, cellular internalization and cytotoxicity were investigated. Regioselective glycosylation patterns and shapes of the NPs could tune the receptors' binding affinity, resulting in precise cellular uptake of AuNPs. Two cell lines HepG2 and HeLa were probed with galactosamine-embedded fluorescent AuNPs, revealing significant differences in cytotoxicity and uptake mechanism in upright and invert in vitro cell-culture assay, high-specificity toward uptake, and allowing for a rapid screening and optimization technique.Keywords
Funding Information
- Indian Institute of Science Education and Research Mohali (BT/PR21934/NNT/28/1242/2017, BT/PR34475/MED/15/210/2020, STARS/APR2019/CS/426/FS, SERB/F/9228/2019–2020)
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