Indigestible cowpea proteins reduced plasma cholesterol after long-term oral administration to Sprague-Dawley rats
Open Access
- 16 June 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Food Production, Processing and Nutrition
- Vol. 3 (1), 1-11
- https://doi.org/10.1186/s43014-021-00061-1
Abstract
Cowpea protein isolate (CPI) was subjected to various dry and wet heat pretreatments followed by sequential digestion with pepsin and pancreatin; the undigested residues were isolated as the indigestible cowpea proteins (ICPs). All the ICPs exhibited in vitro bile acid-binding capacity but ICP from the slow cooling-induced gelation had the highest yield (68%) and was used for rat feeding experiments to determine effect on plasma total cholesterol (TC). Groups consisting of 3 male and 3 female Sprague-Dawley rats each were fed hypercholesterolemic diets that contained casein only or casein that was partially substituted with ICP of CPI for 6 weeks. Results showed diet that contained 5% (w/w) ICP was more effective in preventing TC increase (1.8 mmol/L) when compared to increases of 9.34 and 4.15 mmol/L for CPI and casein only diets, respectively. GraphicalKeywords
Funding Information
- Manitoba Pulse and Soybean Growers
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