Plasma Exosomal miR-146b-5p and miR-222-3p are Potential Biomarkers for Lymph Node Metastasis in Papillary Thyroid Carcinomas
Open Access
- 1 February 2020
- journal article
- research article
- Published by Taylor & Francis Ltd in OncoTargets and Therapy
- Vol. ume 13, 1311-1319
- https://doi.org/10.2147/ott.s231361
Abstract
Background: Lymph node metastasis (LNM) is associated with increased risk of recurrence and poor prognosis in papillary thyroid cancer (PTC). Novel non-invasive biomarkers for the prediction of LNM in PTC patients are still urgently needed. In this study, the relationship between the expression of plasma exosomal microRNAs (miRNAs) and LNM was analyzed. Further, we aimed to explore if exosomal miRNAs can serve as indicators of LNM in PTC patients. Methods: A total of 64 PTC patients who underwent total thyroidectomy and neck dissection from June 2018 to July 2018 in West China Hospital were enrolled in this study. Plasma exosomes were isolated by exoRNeasy Serum/Plasma Maxi Kit. The levels of selected exosomal miRNAs were detected by real-time quantitative PCR (qRT-PCR). Cox proportional hazard analyses and receiver operating characteristic (ROC) curves were conducted to evaluate the predictive efficiency. Furthermore, PTC cell lines with transfection of miRNA mimics/inhibitors were used to verify the functions of exosomal miRNAs. Results: 49 PTC patients with LNM and 15 without LNM were included in the present study. Exosomal miR-146b-5p and miR-222-3p were both significantly upregulated in patients with LNM (P values were 0.008 and 0.015, respectively). ROC analyses revealed that the areas under the curves (AUCs) of miR-146b-5p and miR-222-3p for LNM prediction were 0.811 and 0.834, respectively. Moreover, the AUC increased to 0.895 when the two miRNAs used together. Wound healing assays and transwell assays showed that miR-146b-5p and miR-222-3p significantly enhanced the migration and invasion ability of PTC cells in vitro. Conclusion: Plasma exosomal miR-146b-5p and miR-222-3p could serve as potential biomarkers for LNM in PTC.Keywords
This publication has 39 references indexed in Scilit:
- Expression of miRNAs in Papillary Thyroid Carcinomas Is Associated withBRAFMutation and Clinicopathological Features in Chinese PatientsInternational Journal of Endocrinology, 2013
- Reevaluating the Prognostic Significance of Age in Differentiated Thyroid CancerOtolaryngology -- Head and Neck Surgery, 2012
- A multicenter cohort study of total thyroidectomy and routine central lymph node dissection for cN0 papillary thyroid cancerSurgery, 2011
- MicroRNA miR-146b-5p regulates signal transduction of TGF-β by repressing SMAD4 in thyroid cancerOncogene, 2011
- Lateral cervical lymph node metastases from papillary thyroid carcinoma: Predictive factors of nodal metastasisSurgery, 2011
- MicroRNA Signature Distinguishes the Degree of Aggressiveness of Papillary Thyroid CarcinomaAnnals of Surgical Oncology, 2011
- Thyroid Cancer Epidemiology and Prognostic VariablesClinical Oncology, 2010
- Micro-RNAs in thyroid neoplasms: molecular, diagnostic and therapeutic implicationsJournal of Clinical Pathology, 2009
- Regulatory mechanisms of microRNAs involvement in cancerExpert Opinion on Biological Therapy, 2007
- Integrated 18F‐FDG PET/CT for the initial evaluation of cervical node level of patients with papillary thyroid carcinoma: comparison with ultrasound and contrast‐enhanced CTClinical Endocrinology, 2006