CRISPR Tackles Emerging Viral Pathogens
Open Access
- 26 October 2021
- Vol. 13 (11), 2157
- https://doi.org/10.3390/v13112157
Abstract
Understanding the dynamic relationship between viral pathogens and cellular host factors is critical to furthering our knowledge of viral replication, disease mechanisms and development of anti-viral therapeutics. CRISPR genome editing technology has enhanced this understanding, by allowing identification of pro-viral and anti-viral cellular host factors for a wide range of viruses, most recently the cause of the COVID-19 pandemic, SARS-CoV-2. This review will discuss how CRISPR knockout and CRISPR activation genome-wide screening methods are a robust tool to investigate the viral life cycle and how other class 2 CRISPR systems are being repurposed for diagnostics.Keywords
This publication has 150 references indexed in Scilit:
- Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteriaProceedings of the National Academy of Sciences of the United States of America, 2012
- A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial ImmunityScience, 2012
- Avian influenza virus hemagglutinins H2, H4, H8, and H14 support a highly pathogenic phenotypeProceedings of the National Academy of Sciences of the United States of America, 2012
- CRISPR RNA maturation by trans-encoded small RNA and host factor RNase IIINature, 2011
- Expanding the role of 3-O sulfated heparan sulfate in herpes simplex virus type-1 entryVirology, 2010
- Genome-wide RNAi screen identifies human host factors crucial for influenza virus replicationNature, 2010
- A genome-wide genetic screen for host factors required for hepatitis C virus propagationProceedings of the National Academy of Sciences of the United States of America, 2009
- Virus Population Dynamics and Acquired Virus Resistance in Natural Microbial CommunitiesScience, 2008
- Innate Recognition of VirusesImmunity, 2007
- Mechanisms of gene silencing by double-stranded RNANature, 2004