Direct Measurement of the Stall Torque of the Flagellar Motor in Escherichia coli with Magnetic Tweezers
Abstract: The maximum torque the bacterial flagellar motor generates, the stall torque, is a critical parameter that describes the motor energetics. As the motor operates in equilibrium near stall, from the stall torque one can determine how many protons each torque-generating unit (stator) of the motor passes per revolution and then test whether motor rotation and proton flux are tightly or loosely coupled, which has been controversial in recent years.
Keywords: Flagellar / stall torque / passes / rotation / Tweezers / tightly / loosely / coli
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