Transcriptomics and Alternative Splicing Analyses Reveal Large Differences between Maize Lines B73 and Mo17 in Response to Aphid Rhopalosiphum padi Infestation
Open Access
- 10 October 2017
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Plant Science
Abstract
Maize (Zea mays L.) is a staple crop worldwide with extensive genetic variations. Various insects attack maize plants causing large yield loss. Here, we investigated the responses of maize B73, a susceptible line, and Mo17, a resistant line, to the aphid Rhopalosiphum padi on metabolite and transcriptome levels. R. padi feeding had no effect on the levels of the defensive metabolites benzoxazinoids (Bxs) in either line, and Mo17 contained substantially greater levels of Bxs than did B73. Profiling of the differentially expressed genes revealed that B73 and Mo17 responded to R. padi infestation specifically, and importantly, these two lines showed large gene expression differences even without R. padi herbivory. Correlation analysis identified four transcription factors (TFs) that might account for the high Bx levels in Mo17. Similarly, genome-wide alternative splicing (AS) analyses indicated that both B73 and Mo17 had temporally specific responses to R. padi infestation, and these two lines also exhibited large differences of AS regulation under normal condition, and 340 genes, including 10 TFs, were constantly differentially spliced. This study provides large-scale resource datasets for further studies on the mechanisms underlying maize-aphid interactions, and highlights the phenotypic divergence in defense against aphids among maize varieties.Keywords
This publication has 75 references indexed in Scilit:
- Transcriptional responses of Arabidopsis thaliana to chewing and sucking insect herbivoresFrontiers in Plant Science, 2014
- Additive effects of two quantitative trait loci that confer Rhopalosiphum maidis (corn leaf aphid) resistance in maize inbred line Mo17Journal of Experimental Botany, 2014
- Benzoxazinoid Metabolites Regulate Innate Immunity against Aphids and Fungi in MaizePlant Physiology, 2011
- Genetic variation at bx1 controls DIMBOA content in maizeTheoretical and Applied Genetics, 2009
- Plant Terpenoids: Biosynthesis and Ecological FunctionsJournal of Integrative Plant Biology, 2007
- Molecular and functional diversity of maizeCurrent Opinion in Plant Biology, 2006
- Mechanisms of Alternative Pre-Messenger RNA SplicingAnnual Review of Biochemistry, 2003
- Plant terpenoid synthases: Molecular biology and phylogenetic analysisProceedings of the National Academy of Sciences of the United States of America, 1998
- An Elicitor of Plant Volatiles from Beet Armyworm Oral SecretionScience, 1997
- TOXICITY AND FEEDING DETERRENCY OF HYDROXAMIC ACIDS FROM GRAMINEAE IN SYNTHETIC DIETS AGAINST THE GREENBUG, SCHIZAPHIS GRAMINUMEntomologia Experimentalis et Applicata, 1983