Cytotoxic effects of the synthesized Citrus aurantium peels extract nanoparticles against MDA-MB-231 breast cancer cells

Abstract

Currently, nanoparticle is at fastest pace due to the enhanced implication in drug delivery system. The synthesis of chitosan nanoparticles through ionic gelation methods is becoming increasingly relevant due to the low cost involved and environment-friendly approach. In this study, cytotoxic effect of chitosan-Citrus aurantium peels extract nanoparticles (NPs-CA) against MDA-MB-231 breast cancer cells was investigated. The initial confirmations of NPs-CA were using Particles size analysis (PSA), which showed the size of NPs-CA. The Zeta potential was measured to check the stability of NPs-CA. The cytotoxic effect was carried out in MDA-MB-231 breast cancer cells under MTT assay. Based on PSA analysis, the size of F1 and F2 were ranging between 372 – 4472 nm and 94 – 373 nm, respectively. The zeta potential mean value of F2 NPs-CA 34,9 mV, it can be concluded that the particles show less chances for agglomeration. On the various test concentration, the F2 NPs-CA performed better cytotoxic effects than Citrus aurantium peels extract with IC₅₀ value of 83 μg/mL and 320 μg/mL, respectively. These findings pose a positive impact to expand further studies in the development of nanoparticle to cancer therapy.