In Vitro Testing of a Polylactic Polymer Synthesized from Whey
- 3 August 2018
- journal article
- research article
- Published by Babes-Bolyai University in Studia Universitatis Babeș-Bolyai Chemia
- Vol. 63 (2), 93-104
- https://doi.org/10.24193/subbchem.2018.2.09
Abstract
The main purpose of this research is in vitro biocompatibility testing of a polylactic acid polymer (PLA) synthesized by the condensation of lactic acid separated from whey (Zonar). The experimental PLA obtained was characterized by: Scanning Electron Microscopy which pointed a homogeneous microstructure of the polymer without a separation phase and differential scanning calorimetry (DSC) analysis which indicated a melting point of 174(degrees)C of the biomaterial. The cytotoxicity test for experimentally obtained polymer was performed according to ISO 10 993 - 12(3). Six solutions of different concentrations were prepared (30 mg/ml, 20 mg/ml, 10 mg/ml, 5 mg/ml, 2.5 mg/ml and 1.25 mg/ml) and were tested on a fibroblast culture at 48 hours using MTT cell viability and proliferation assay. At 48h the obtained polylactic polymer showed good compatibility with an IC 50 of 64.82 mg/ml. The results showed that the cytotoxicity level can be influenced by the period of time in which the extract acts on the cell culture.Keywords
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