Detection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology
Open Access
- 9 June 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 11 (1), 1-11
- https://doi.org/10.1038/s41598-021-91648-8
Abstract
In the management of diffuse gliomas, the identification and removal of tumor at the infiltrative margin remains a central challenge. Prior work has demonstrated that fluorescence labeling tools and radiographic imaging are useful surgical adjuvants with macroscopic resolution. However, they lose sensitivity at the tumor margin and have limited clinical utility for lower grade histologies. Fiber-laser based stimulated Raman histology (SRH) is an optical imaging technique that provides microscopic tissue characterization of unprocessed tissues. It remains unknown whether SRH of tissues taken from the infiltrative glioma margin will identify microscopic residual disease. Here we acquired glioma margin specimens for SRH, histology, and tumor specific tissue characterization. Generalized linear mixed models were used to evaluate agreement. We find that SRH identified residual tumor in 82 of 167 margin specimens (49%), compared to IHC confirming residual tumor in 72 of 128 samples (56%), and H&E confirming residual tumor in 82 of 169 samples (49%). Intraobserver agreements between all 3 modalities were confirmed. These data demonstrate that SRH detects residual microscopic tumor at the infiltrative glioma margin and may be a promising tool to enhance extent of resection.Keywords
Funding Information
- National Institute of Neurological Disorders and Stroke (1K08NS110919-01)
- Robert Wood Johnson Foundation (74259)
This publication has 24 references indexed in Scilit:
- Extent of resection in patients with glioblastoma: limiting factors, perception of resectability, and effect on survivalJournal of Neurosurgery, 2012
- Multicolored stain-free histopathology with coherent Raman imagingLaboratory Investigation, 2012
- A Sensitive and Specific Diagnostic Panel to Distinguish Diffuse Astrocytoma From Astrocytosis: Chromosome 7 Gain With Mutant Isocitrate Dehydrogenase 1 and p53Journal of Neuropathology and Experimental Neurology, 2011
- Characterization of R132H Mutation‐specific IDH1 Antibody Binding in Brain TumorsBrain Pathology, 2009
- Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering MicroscopyScience, 2008
- Malignant Gliomas in AdultsThe New England Journal of Medicine, 2008
- Role of Extent of Resection in the Long-Term Outcome of Low-Grade Hemispheric GliomasJournal of Clinical Oncology, 2008
- Fluorescence-guided surgery with 5-aminolevulinic acid for resection of malignant glioma: a randomised controlled multicentre phase III trialThe Lancet Oncology, 2006
- p53 in Nonneoplastic Central Nervous System Lesions: An Immunohistochemical and Genetic Sequencing StudyNeurosurgery, 2002
- Changes in Proliferating Cell Nuclear Antigen Expression in Glioblastoma Multiforme Cells along a Stereotactic Biopsy TrajectoryNeurosurgery, 1994