Lactobacillus brevis Lipase: Purification, Immobilization onto Magnetic Florosil NPs, Characterization and Application as a Detergent Additive
- 8 May 2017
- journal article
- research article
- Published by Walter de Gruyter GmbH in Tenside Surfactants Detergents
- Vol. 54 (3), 194-205
- https://doi.org/10.3139/113.110495
Abstract
In this study, a thermo-tolerant and alkaline lipase enzyme was purified from Lactobacillus brevis and immobilized onto modified γ-Fe3O4 florisil nanoparticles (γ-Fe3O4 MF NFs) and the usability of free lipase (FL) and immobilized lipases (IML) as detergent additives was investigated. Lipase enzyme was purified by fractional precipitation using 20% ammonium sulfate, DEAE-Sephadex ion-exchange chromatographic column, and Sephacryl S200 gel filtration chromatographic techniques. Then, the enzyme was purified, which resulted in 135.2-fold purification. Its molecular mass was determined to be 57 kDa by SDS-PAGE. The covalent immobilization of purified lipase was done using γ-Fe3O4 MF NPs. γ-Fe3O4 MF NPs and IML were characterized by using SEM, TEM, FT-IR, and XRD. IML showed a good thermo-stability and its activities were calculated as 80% at 60°C. The free and IML enzymes were most stable at alkaline pHs in the range of 7.0–10.0. Also, IML is more stable towards metal ions compared to free lipase enzyme. Washing performances of some detergent formulations were investigated in the presence and absence of Lipase. Olive oil was removed by the detergent alone and by the detergent and IML at ratios of 45% and 72%, respectively. The study on removal of oil stain from cotton cloths indicated that the removal of oil was superior in the presence of IML and IML with detergent, when compared to the detergent alone.Keywords
This publication has 51 references indexed in Scilit:
- Cs+ Removal from Aqueous Solutions through Adsorption onto Florisil® Impregnated with Trihexyl(tetradecyl)phosphonium ChlorideMolecules, 2013
- Evaluation of a New Lipase fromStaphylococcussp. for Detergent Additive CapabilityBioMed Research International, 2013
- Enhanced degradation of trichloroethylene in nano-scale zero-valent iron Fenton system with Cu(II)Journal of Hazardous Materials, 2012
- Degradation characteristics of humic acid over iron oxides/Fe0 core–shell nanoparticles with UVA/H2O2Journal of Hazardous Materials, 2010
- An effect of iron(III) oxides crystallinity on their catalytic efficiency and applicability in phenol degradation—A competition between homogeneous and heterogeneous catalysisApplied Catalysis A: General, 2009
- Effective removal of AB24 dye by nano/micro-size zero-valent ironSeparation and Purification Technology, 2008
- Applications of magnetic nanoparticles in biomedicineJournal of Physics D: Applied Physics, 2003
- Production and properties of an alkaline, thermophilic lipase from Pseudomonas fluorescens NS2WJournal of Industrial Microbiology & Biotechnology, 2002
- Lipase from Ralstonia pickettii as an additive in laundry detergent formulationsProcess Biochemistry, 2000
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970