The Influence of Preanalytical Biospecimen Handling on the Measurement of B Vitamers, Amino Acids, and Other Metabolites in Blood
- 9 January 2023
- journal article
- research article
- Published by Mary Ann Liebert Inc in Biopreservation and Biobanking
- Vol. 21 (5), 467-476
- https://doi.org/10.1089/bio.2022.0053
Abstract
Introduction: Sample handling can influence biomarker measurement and introduce variability when combining data from multiple studies or study sites. To inform the development of blood collection protocols within a multisite cohort study, we directly quantified concentrations of 54 biomarkers in blood samples subjected to different handling conditions. Materials and Methods: We obtained serum, lithium heparin plasma, and EDTA plasma from 20 adult volunteers. Tubes of chilled whole blood were either centrifuged and processed within 2 hours of collection (the “reference standard”) or were stored with cool packs for 24 or 48 hours; centrifuged before and/or after this delay; or collected in tubes with/without gel separators. We used linear mixed models with random intercepts to estimate geometric mean concentrations and relative percent differences across the conditions. Results: Compared to the reference standard tubes, concentrations of many biomarkers changed after processing delays, but changes were often small. In serum, we observed large differences for B vitamers, glutamic acid (37% and 73% increases with 24- and 48-hour delays, respectively), glycine (12% and 23% increases), serine (16% and 27% increases), and acetoacetate (−19% and −26% decreases). Centrifugation timing and separator tube use did not affect concentrations of most biomarkers. Conclusion: Sample handling should be consistent across samples within an analysis. The length of processing delays should be recorded and accounted for when this is not feasible.Keywords
This publication has 29 references indexed in Scilit:
- Most Blood Biomarkers Related to Vitamin Status, One-Carbon Metabolism, and the Kynurenine Pathway Show Adequate Preanalytical Stability and Within-Person Reproducibility to Allow Assessment of Exposure or Nutritional Status in Healthy Women and Cardiovascular PatientsJournal of Nutrition, 2014
- Targeted Metabolomics Identifies Reliable and Stable Metabolites in Human Serum and Plasma SamplesPLOS ONE, 2014
- Quality Markers Addressing Preanalytical Variations of Blood and Plasma Processing Identified by Broad and Targeted Metabolite ProfilingClinical Chemistry, 2014
- Measurement of nitric oxide-related amino acids in serum and plasma: Effects of blood clotting and type of anticoagulantClinica Chimica Acta; International Journal of Clinical Chemistry, 2013
- Kinetic Modeling of Storage Effects on Biomarkers Related to B Vitamin Status and One-Carbon MetabolismClinical Chemistry, 2012
- Differences between Human Plasma and Serum Metabolite ProfilesPLOS ONE, 2011
- β-Elemene Piperazine Derivatives Induce Apoptosis in Human Leukemia Cells through Downregulation of c-FLIP and Generation of ROSPLOS ONE, 2011
- Differences in metabolite profile between blood plasma and serumAnalytical Biochemistry, 2010
- Quantitative profiling of biomarkers related to B‐vitamin status, tryptophan metabolism and inflammation in human plasma by liquid chromatography/tandem mass spectrometryRapid Communications in Mass Spectrometry, 2009
- Automated Assay for the Determination of Methylmalonic Acid, Total Homocysteine, and Related Amino Acids in Human Serum or Plasma by Means of Methylchloroformate Derivatization and Gas Chromatography–Mass SpectrometryClinical Chemistry, 2005