A simple method for long-term recording of the electrocardiogram in prawns
- 1 January 2021
- journal article
- research article
- Published by Inter-Research Science Center in Aquatic Biology
- Vol. 30, 59-68
- https://doi.org/10.3354/ab00741
Abstract
This paper presents a novel approach to the long-term recording of electrocardiogram (ECG) signals in prawns, including a 3-lead electrode, a lightweight external wires assembly, a waterproof electrode-wire junction, and a standardized implanting procedure. The proposed low-cost device is easily constructed, even by untrained undergraduate students, using common laboratory materials. Consistent ECG recordings were obtained over a period of 72 h in experiments on 6 freely moving prawns (Macrobrachium rosenbergii). A subsequent experiment conducted continuously for 38 d revealed that despite gradual attenuation the ECG signals could still be identified (signal-to-noise ratio >= 3). The prawns survived implantation and later underwent natural molting, whereupon the electrode was successfully re-implanted. The proposed electrode is a valuable tool by which to gain insight into the long-term physiological state of crustaceans.Keywords
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