Fine mapping and candidate gene analysis of dwarf gene Rht14 in durum wheat (Triticum durum)
- 4 January 2022
- journal article
- research article
- Published by Springer Science and Business Media LLC in Functional & Integrative Genomics
- Vol. 22 (2), 141-152
- https://doi.org/10.1007/s10142-021-00825-5
Abstract
Semi-dwarf and dwarf genes were widely used in wheat breeding for improving lodging resistant and increasing yield. Rht14 dwarf gene was identified and deployed in durum wheat, where it showed advantage on important agronomic potential. The reciprocal F2 populations derived of Castelporziano (CP) and Langdon (L) were used for mapping of Rht14, which was located in intervals 4.8 cM and 10.38 cM by KASP (Kompetitive Allele Specific PCR) markers, respectively, where corresponding to 312–454 Mbp on chromosome 6A, and finally, it was mapped to the genomic region of 402 ~ 408 Mbp in Durum Wheat Svevo RefSeq Rel. 1.0 (i.e., 405 ~ 411 Mbp in Chinese Spring RefSeq v.1.0) using recombinants by indel markers. The expression of TdGA2oxA9 was higher in dwarf line than tall lines and the bioactive GA1 was lower. No sequence difference was observed in the promoter and coding region of GA2oxA9 between the dwarf and tall parent, while obvious DNA methylation difference was found in its promoter. Two methylation-related genes with high confidence located in the candidate region and expressed differently between the tall and dwarf ones. This study proposed that Rht14 might regulate the expression of GA2oxA9 by DNA methylation in its promoter, which provided a way to clone Rht14 and to further investigate the mechanism behind.Funding Information
- Natural Science Foundation of China (31671695)
- Sub-project of the 863 Program of the Ministry of Science and Technology (2013AA102902)
- China 111 Project (B12007)
- Australian Centre for International Agricultural Research (CIM/2005/111)
- National Natural Science Foundation of China (31501307)
- China Postdoctoral Science Foundation (2017M623259)
- Natural Science Foundation of Shaanxi Province (2017JM3022)
- General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
This publication has 38 references indexed in Scilit:
- Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivarsProceedings of the National Academy of Sciences of the United States of America, 2013
- Identification and Characterization of an Epi-Allele of FIE1 Reveals a Regulatory Linkage between Two Epigenetic Marks in RiceTHE PLANT CELL ONLINE, 2012
- Molecular Characterization ofRht-1Dwarfing Genes in Hexaploid WheatPlant Physiology, 2011
- Dominant and Pleiotropic Effects of aGAIGene in Wheat Results from a Lack of Interaction between DELLA and GID1Plant Physiology, 2011
- A metastable DWARF1 epigenetic mutant affecting plant stature in riceProceedings of the National Academy of Sciences of the United States of America, 2009
- A Novel Class of Gibberellin 2-Oxidases Control Semidwarfism, Tillering, and Root Development in RiceTHE PLANT CELL ONLINE, 2008
- Gibberellin Metabolism and its RegulationAnnual Review of Plant Biology, 2008
- Plant SET domain-containing proteins: Structure, function and regulationBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 2007
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- THE ESTIMATION OF MAP DISTANCES FROM RECOMBINATION VALUESAnnals of Eugenics, 1943