| Rhipicephalus sanguineus, the brown dog tick, is the most widespread tick in the world and transmits broad range of pathogens to dogs. The aim of this study was to identify the tick species infested in dogs by using a combination of LM and molecular analysis. A total of 237 tick samples were collected from dogs and identified by using Light Microscope (LM) and molecular technique. Morphological identification revealed that all of tick infestation in dogs was R. sanguineus spp. and 16SrRNA gene of R. sanguineus species sequences analyses proved 100% sequences identitieswhen compared with the reference data in Genbank records. The present studies confirm R. sanguineus is common tick species infesting in dogs within Nay Pyi Taw area.