Physiological Basis for the Tolerance of Yeast Zygosaccharomyces bisporus to Salt Stress
Open Access
- 1 October 2017
- journal article
- Published by Bogor Agricultural University in HAYATI Journal of Biosciences
- Vol. 24 (4), 176-181
- https://doi.org/10.1016/j.hjb.2017.11.001
Abstract
Zygosaccharomyces bisporus is a moderately halotolerant yeast isolated from highly sugary and salty foods. We performed various evident biochemical and in vivo experiments as first of its kind to sketch out the possible overlay of salt tolerance mechanism in this model organism. The growth and survival curve analysis revealed that 1.0 M NaCl concentration (sublethal) enacts growth inhibitory effects with prompting immediate delay in cell division cycle; however, yeast cells adopted modified stress physiologically with further stretched stress spans which was accompanied by an upsurge in the level of cellular metabolites such as trehalose (reserve carbohydrate) and chiefly glycerol (polyols) as major compatible osmolytes, suggesting their role in defense mechanism against osmotic stress. To further elucidate the relation of osmotic stress cell physiology to salinity, thiobarbituric acid reactive substances, protein carbonyl, and reduced glutathione content were measured in salt-stressed cells demonstrating positive correlation of reactive oxygen species generation in Z. bisporus with an elevated concentration of lipid and protein oxidation, thereby damaging cell membrane and eventually causing cell death. We assessed NaCl exposure sourcing increased intracellular reactive oxygen species concentration, by an electron transfer-based colorimetric cupric-reducing antioxidant capacity assay justifying that cellular total antioxidant capacity which uses all the combined antioxidant activities present within vitamins, proteins, lipids, and glutathione reverses these deleterious stress effects. Henceforth, performance of Z. bisporus MTCC 4801 mounted because of stress regime seems to be multifactorialThis publication has 31 references indexed in Scilit:
- Zygosaccharomyces sapae sp. nov., isolated from Italian traditional balsamic vinegarInternational Journal of Systematic and Evolutionary Microbiology, 2013
- Alkali Metal Cation Transport and Homeostasis in YeastsMicrobiology and Molecular Biology Reviews, 2010
- The salt tolerant yeast Zygosaccharomyces rouxii possesses two plasma-membrane Na+/H+-antiporters (ZrNha1p and ZrSod2–22p) playing different roles in cation homeostasis and cell physiologyFungal Genetics and Biology, 2008
- Cloning and characterization of genes encoding trehalose-6-phosphate synthase (TPS1) and trehalose-6-phosphate phosphatase (TPS2) from Zygosaccharomyces rouxiiFEMS Yeast Research, 2003
- Two putative MAP kinase genes, ZrHOG1 and ZrHOG2, cloned from the salt-tolerant yeast Zygosaccharomyces rouxii are functionally homologous to the Saccharomyces cerevisiae HOG1 geneMicrobiology, 1999
- Efflux of sodium ions by a Na+/H+-antiporter during salt stress in the salt-tolerant yeast Zygosaccharomyces rouxii.The Journal of General and Applied Microbiology, 1995
- The water relations of growth and polyhydroxy alcohol production by ascomycetous yeastsJournal of General Microbiology, 1993
- Living with Water Stress: Evolution of Osmolyte SystemsScience, 1982
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- THE CHEMISTRY OF INSECT HEMOLYMPHThe Journal of general physiology, 1957