Formation and Investigation of Physicochemical, Biological and Bacteriostatic Properties of Nanocomposite Foils Containing Silver Nanoparticles and Graphene Oxide in Hyaluronic Acid Matrix
Open Access
- 18 June 2021
- Vol. 14 (12), 3377
- https://doi.org/10.3390/ma14123377
Abstract
Natural polysaccharides, including hyaluronic acid, find a wide range of applications in biomedical sciences. There is a growing interest in nanocomposites containing hyaluronic acid and nanoparticles such as nanometals or graphene. In this study, we prepared foils of pure sodium hyaluronate and sodium hyaluronate containing nanosilver, graphene oxide, nanosilver/graphene oxide and characterized their properties. UV-vis spectroscopy and scanning electron microscopy (SEM) confirmed the formation of 10–20 nm silver nanoparticles. The structural changes were investigated using Fourier transforms infrared (FTIR) spectra and size exclusion chromatography. The obtained results suggest changes in molecular weights in the samples containing nanoparticles, which was highest in a sample containing nanosilver/graphene oxide. We also assessed the mechanical properties of the foils (thickness, tensile strength and elongation at break) and their wettability. The foils containing nanosilver and nanosilver/graphene oxide presented bacteriostatic activity against E. coli, Staphylococcus spp. and Bacillus spp., which was not observed in the control and sample containing graphene oxide. The composites containing graphene oxide and nanosilver/graphene oxide exhibited a cytotoxic effect on human melanoma WM266-4 cell lines (ATCC, Manassas, VA, USA).Funding Information
- Narodowym Centrum Nauki (2016/21/B/ST8/02107)
- Ministerstwo Nauki i Szkolnictwa Wyższego (subsidy of the Ministry of Science and Higher Education for the University of Agriculture in Krakow for 2020)
This publication has 51 references indexed in Scilit:
- Chemical Modification of PolysaccharidesISRN Organic Chemistry, 2013
- Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteriaNanomedicine: Nanotechnology, Biology and Medicine, 2012
- Chitosan‐Functionalized Graphene Oxide as a Nanocarrier for Drug and Gene DeliverySmall, 2011
- A biotechnological perspective on the application of iron oxide magnetic colloids modified with polysaccharidesBiotechnology Advances, 2011
- Polymer-Nanoparticle Composites: From Synthesis to Modern ApplicationsMaterials, 2010
- Hyaluronan- and heparin-reduced silver nanoparticles with antimicrobial propertiesNanomedicine, 2009
- PEGylated Nanographene Oxide for Delivery of Water-Insoluble Cancer DrugsJournal of the American Chemical Society, 2008
- Synthesis of Water Soluble GrapheneNano Letters, 2008
- Review. Hyaluronan: A Powerful Tissue Engineering ToolTissue Engineering, 2006
- Estimation of the surface free energy of polymersJournal of Applied Polymer Science, 1969