Rapid molecular detection and isolation of canine distemper virus in naturally infected dogs
- 30 December 2022
- journal article
- research article
- Published by Veterinary Journal of Ankara University in Ankara Üniversitesi Veteriner Fakültesi Dergisi
- Vol. 70 (1), 49-56
- https://doi.org/10.33988/auvfd.846475
Abstract
The Canine Distemper Virus (CDV), which infects dogs and a broad range of animal species, remains a serious concern in Turkey as well as worldwide. The current study shows that CDV can be detected and isolated rapidly and specifically in naturally infected dogs. The samples used included whole blood, nasal swab, ocular swab, rectal swab, and urine (n = 250) and were collected from 50 stray dogs, which had not received any vaccine against CDV in 2016–17. The presence of the CDV genome was confirmed in 105 (42%) samples using one-Step real-time RT-PCR. In total, 39 dogs were diagnosed with CDV infection based on the detection of characteristic cytopathic effects (CPE) in MDCK cells (Madin-Darby Canine Kidney), which was further verified by the fluorescence antibody technique (FAT). A total of 12 one-Step real-time RT-PCR negative samples, consisting of 4 rectal swabs and 8 urine samples, were found to be positive by virus isolation.In the tests for viral genome detection and virus isolation, positive correlations were found for blood samples, nasal swabs, ocular swabs (p<0.01, r = 1), rectal swabs (p<0.01, r = 0.844), and urine samples (p<0.01, r = 0.697).Out of a total of 117 (46.8%) samples showing viral growth in cell culture, 37 (31.62%) were found to have a high CPE, 26 (22.23%) had medium CPE, and 54 (46.15%) had low CPE. The highest CPE levels detected by FAT were for rectal swab and urine samples. In conclusion, the one-Step real-time RT-PCR method on rectal swab samples proved to be a highly sensitive method for the rapid and reliable detection of CDV. Besides, non-modified MDCK cell cultures are better for the isolation of CDV from naturally infected dogs.Keywords
Funding Information
- Burdur Mehmet Akif Ersoy Üniversitesi Bilimsel Araştırma ve Projeler Koordinatörlüğü (0403-DR-16)
This publication has 39 references indexed in Scilit:
- Comparison of two assays for detection of antibodies against canine parvovirus and canine distemper virus in dogs admitted to a Florida animal shelterJournal of the American Veterinary Medical Association, 2012
- Pathogenesis and phylogenetic analyses of canine distemper virus strain ZJ7 isolate from domestic dogs in ChinaVirology Journal, 2011
- Rectal swabs can be used for diagnosis of viral gastroenteritis with a multiple real-time PCR assayJournal of Clinical Virology, 2011
- Pathogenesis and immunopathology of systemic and nervous canine distemperVeterinary Immunology and Immunopathology, 2009
- Comparative analyses of canine distemper viral isolates from clinical cases of canine distemper in vaccinated dogsVeterinary Microbiology, 2006
- Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitisResearch in Veterinary Science, 2006
- Canine distemper virus-induced depletion of uninfected lymphocytes is associated with apoptosisVeterinary Immunology and Immunopathology, 2005
- A canine distemper virus epidemic in Serengeti lions (Panthera leo)Nature, 1996
- Control of canine distemperVeterinary Microbiology, 1995
- Pathogenicity of morbilliviruses for terrestrial carnivoresVeterinary Microbiology, 1995