Glutathione S transferase and catalase gene polymorphisms did not tend to influence the severity of hemoglobin E/β-thalassemia
Open Access
- 31 January 2020
- journal article
- research article
- Published by Universa Medicina in Universa Medicina
- Vol. 39 (1), 19-26
- https://doi.org/10.18051/univmed.2020.v39.19-26
Abstract
Background Thalassemia, a monogenic genetic disease of red blood cells, is spread widely throughout the world. Glutathione S transferase (GST) enzymes have an antioxidant role in detoxification processes of toxic substances This study aimed to determine the role of the genetic modifier genes GSTT1 and GSTM1, and the catalase (CAT) gene in clinical degrees of hemoglobin (Hb)E/β thalassemia. Methods Sixty HbE/β Thalassemia patients were examined to determine their clinical pictures. Clinical score was based on age when thalassemia symptoms appeared, time of diagnosis, time of first blood transfusion, pre-transfusion hemoglobin concentration, frequency of transfusions, and enlargement of spleen. Ferritin concentration was also obtained from medical records. Gene polymorphisms of GSTT1, GSTM1, and CAT were measured using PCR and PCR-RFLP methods. Clinical scores were categorized into mild (0-3.5), moderate (4-7), and severe (7.5-10) degrees, while ferritin level was expressed in mg/dL. One way Anova was used to analyze the data. Results The clinical appearance showed that severe, moderate, and mild degrees accounted for 42%, 45%, and 13%, respectively. The majority had a high ferritin level of more than 5000 mg/dL (67%). GSTT1 null, GSTM1 null, and CAT minor allele genotypes were 21.7%, 33.3%, and 12.1%, respectively. GSTT1, GSTM1, and CAT genotypes had no impact on the severity of thalassemia patients (p=0.091, p=0.082, and p=0.141, respectively). Conclusion GSTT1, GSTM1, CAT gene polymorphisms tend to be a minor aspect of severity of clinical outcome for HbE/â thalassemia patients and should be not considered a routine laboratory check.Keywords
This publication has 22 references indexed in Scilit:
- Cardiac iron overload in sickle-cell diseaseAmerican Journal of Hematology, 2014
- Inborn defects in the antioxidant systems of human red blood cellsFree Radical Biology & Medicine, 2014
- A simple method for examination of polymorphisms of catalase exon 9: rs769217 in Hungarian microcytic anemia and beta-thalassemia patientsArchives of Biochemistry and Biophysics, 2012
- Effects of rs769217 and rs1001179 polymorphisms of catalase gene on blood catalase, carbohydrate and lipid biomarkers in diabetes mellitusFree Radical Research, 2012
- Recent Advances in β-ThalassemiasPediatric Reports, 2011
- A Deletion Polymorphism in Glutathione-S-Transferase Mu (GSTM1) and/or Theta (GSTT1) Is Associated with an Increased Risk of Toxicity after Autologous Blood and Marrow TransplantationTransplantation and Cellular Therapy, 2010
- Beta-thalassemiaGenetics in Medicine, 2010
- Glutathione S-transferase gene deletions and their effect on iron status in HbE/β thalassemia patientsAnnals of Hematology, 2009
- Glutathione S‐transferase gene polymorphism and cardiac iron overload in thalassaemia majorBritish Journal of Haematology, 2008
- A scoring system for the classification of β‐thalassemia/Hb E disease severityAmerican Journal of Hematology, 2008