Dynamic and widespread control of poly(A) tail length during macrophage activation
Open Access
- 5 May 2022
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in RNA
- Vol. 28 (7), 947-971
- https://doi.org/10.1261/rna.078918.121
Abstract
The poly(A) tail enhances translation and transcript stability, and tail length is under dynamic control during cell state transitions. Tail regulation plays essential roles in translational timing and fertilization in early development, but poly(A) tail dynamics have not been fully explored in post-embryonic systems. Here, we examined the landscape and impact of tail length control during macrophage activation. Upon activation, more than 1,500 mRNAs, including pro-inflammatory genes, underwent distinctive changes in tail lengths. Increases in tail length correlated with mRNA levels regardless of transcriptional activity, and many mRNAs that underwent tail extension encode proteins necessary for immune function and post-transcriptional regulation. Strikingly, we found that ZFP36, whose protein product destabilizes target transcripts, undergoes tail extension. Our analyses indicate that many mRNAs undergoing tail lengthening are, in turn, degraded by elevated levels of ZFP36, constituting a post-transcriptional feedback loop that ensures transient regulation of transcripts integral to macrophage activation. Taken together, this study establishes the complexity, relevance and widespread nature of poly(A) tail dynamics, and the resulting post-transcriptional regulation during macrophage activation.Keywords
Funding Information
- Discretionary funds
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