Integration of transcriptomics and metabolomics to reveal the effect of ginsenoside Rg3 on allergic rhinitis in mice
Open Access
- 24 January 2023
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Food & Function
- Vol. 14 (5), 2416-2431
- https://doi.org/10.1039/d2fo03885d
Abstract
Increasing studies have demonstrated that ginsenoside Rg3 (Rg3) plays an important role in the prevention and treatment of various diseases, including allergic lower airway inflammation such as asthma. To investigate the role of Rg3 in allergic upper airway disease, the effect and therapeutic mechanism of Rg3 in allergic rhinitis (AR) were studied. Ovalbumin-induced AR model mice were intragastrically administered with Rg3. Nasal symptoms, levels of IgE, IL-4, IL-5, IL-13, SOD and MDA in serum, and histopathological analysis of nasal mucosa were used to evaluate the effect of Rg3 on ameliorating AR in mice. Moreover, nasal mucosa samples from normal control group, AR model group and high dosage of Rg3 were collected to perform omics analysis. The differentially expressed genes and significantly changed metabolites were screened based on transcriptomics and metabolomics analyses, respectively. Integrative analysis was further performed to confirm the hub genes, metabolites and pathways. After Rg3 intervention, the nasal symptoms and inflammatory infiltration were effectively improved, the levels of IgE, IL-4, IL-5, IL-13 and MDA were significantly reduced, and the level of SOD was obviously increased. The results of the qRT‒PCR assay complemented the transcriptomic findings. The integrated analysis showed that Rg3 played an anti-AR role mainly by regulating the interaction network, which was constructed by 12 genes, 8 metabolites and 4 pathways. Our findings suggested that Rg3 had a therapeutic effect on ovalbumin-induced AR in mice by inhibiting inflammation development and reducing oxidative stress. The present study could provide a potential natural agent for the treatment of AR.Keywords
Funding Information
- Natural Science Foundation of Jilin Province (20200201200JC, 20200201411JC, 20200201600JC)
- National Natural Science Foundation of China (81870701, 82071016)
This publication has 82 references indexed in Scilit:
- S100A8 Modulates Mast Cell Function and Suppresses Eosinophil Migration in Acute AsthmaAntioxidants and Redox Signaling, 2011
- Isolation and analysis of ginseng: advances and challengesNatural Product Reports, 2011
- 5A, an Apolipoprotein A-I Mimetic Peptide, Attenuates the Induction of House Dust Mite-Induced AsthmaThe Journal of Immunology, 2011
- Sputum dipalmitoylphosphatidylcholine level as a novel airway inflammatory marker in asthmatic childrenThe Clinical Respiratory Journal, 2009
- Ozone, Oxidant Defense Genes, and Risk of Asthma during AdolescenceAmerican Journal of Respiratory and Critical Care Medicine, 2008
- The collagen family members as cell adhesion proteinsBioEssays, 2007
- Protective Effects of Increasing Vitamin E and A Doses on Cisplatin-Induced Oxidative Damage to Kidney Tissue in RatsUrologia Internationalis, 2005
- Female mice are more susceptible to the development of allergic airway inflammation than male miceClinical & Experimental Allergy, 2005
- Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction NetworksGenome Research, 2003
- Antigen-induced generation of lyso-phospholipids in human airways.The Journal of Experimental Medicine, 1996