3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model
Open Access
- 8 March 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Scientific Reports
- Vol. 11 (1), 1-15
- https://doi.org/10.1038/s41598-021-84123-x
Abstract
Bone regeneration is a complex process and the clinical translation of tissue engineered constructs (TECs) remains a challenge. The combination of biomaterials and mesenchymal stem cells (MSCs) may enhance the healing process through paracrine effects. Here, we investigated the influence of cell format in combination with a collagen scaffold on key factors in bone healing process, such as mineralization, cell infiltration, vascularization, and ECM production. MSCs as single cells (2D-SCs), assembled into microtissues (3D-MTs) or their corresponding secretomes were combined with a collagen scaffold and incubated on the chicken embryo chorioallantoic membrane (CAM) for 7 days. A comprehensive quantitative analysis was performed on a cellular level by histology and by microcomputed tomography (microCT). In all experimental groups, accumulation of collagen and glycosaminoglycan within the scaffold was observed over time. A pronounced cell infiltration and vascularization from the interface to the surface region of the CAM was detected. The 3D-MT secretome showed a significant mineralization of the biomaterial using microCT compared to all other conditions. Furthermore, it revealed a homogeneous distribution pattern of mineralization deposits in contrast to the cell-based scaffolds, where mineralization was only at the surface. Therefore, the secretome of MSCs assembled into 3D-MTs may represent an interesting therapeutic strategy for a next-generation bone healing concept.Funding Information
- Hartmann Müller Stifung Zürich (180)
This publication has 94 references indexed in Scilit:
- Hyaluronic acid based scaffolds for tissue engineering—A reviewCarbohydrate Polymers, 2013
- 3D mesenchymal stem/stromal cell osteogenesis and autocrine signallingBiochemical and Biophysical Research Communications, 2012
- Angiogenesis in bone regenerationInjury, 2011
- Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffoldBiomaterials, 2011
- The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitorsNature Materials, 2010
- Effects of single wall carbon nanotubes and its functionalization with sodium hyaluronate on bone repairLife Sciences, 2010
- The Chick Chorioallantoic Membrane: A Model of Molecular, Structural, and Functional Adaptation to Transepithelial Ion Transport and Barrier Function during Embryonic DevelopmentJournal of Biomedicine and Biotechnology, 2010
- Regulation of Osteogenesis-Angiogenesis Coupling by HIFs and VEGFJournal of Bone and Mineral Research, 2009
- Osteoinduction, osteoconduction and osseointegrationEuropean Spine Journal, 2001
- Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based TherapiesTissue Engineering, 2001