A pot experiment was carried out at the Experimental Farm, Faculty of Agriculture, South Valley University, Qena, Egypt during the 2015-2016 and 2016-2017 seasons to study the effect of nitrogen fertilizer levels and types of irrigation water (W1; Tap water only, W2; manipulated wastewater with sandy filter, W3; manipulated wastewater with nano-titanium dioxide + sandy filter and W4; a mixture of treated wastewater with nano-titanium dioxide and sandy filter + tap water) on growth, yield and its attributes of barley cv. Giza 2000. A randomized complete block design (RCBD) using a split-plot arrangement with four replications was used. The main plot was four different levels of nitrogen fertilizer and the sub-plot were four different types of irrigation water. The seeds of above mentioned variety were used in the pot experiments. The highest mean values of plant height, number of tillers plant-1, spike length, number of grains spike-1, spike weight, biological yield plant-1 and grain yield plant-1 were obtained from high level of nitrogen (60 kg N fed-1). Also, the highest values of above traits were obtained from irrigation with treated wastewater with nano-titanium dioxide and sandy filter + tap water (W4). It could be concluded that under the conditions of the experiment, application of high levels of nitrogen (N60) under irrigation with treated wastewater with nano-titanium dioxide and sandy filter+ tap water (W4) is recommended.