Preparation, statistical optimisation andin vitrocharacterisation of poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/poly (lactic-co-glycolic acid) blend nanoparticles for prolonged delivery of teriparatide
- 3 July 2016
- journal article
- research article
- Published by Taylor & Francis Ltd in Journal of Microencapsulation
- Vol. 33 (5), 460-474
- https://doi.org/10.1080/02652048.2016.1208296
Abstract
The purpose of this study was the preparation, optimisation and in vitro characterisation of PHBV and PLGA blend nanoparticles (NPs) for prolonged delivery of Teriparatide. Double emulsion solvent evaporation technique was employed for the fabrication of NPs. The nanoformulation was optimised using the Box–Behnken methodology. The morphological properties of NPs were assessed by both SEM and transmission electron microscopy (TEM). Encapsulation of Teriparatide within the NPs and lacking of chemical bonds between drug and copolymers were proved by XRPD, FTIR and DSC. The structural stability of Teriparatide after processing was confirmed by fluorescence spectrometry. The average size of optimised NPs was 250.0 nm with entrapment efficiency (EE) of 89.5% and drug loading (DL) of 5.0%. Teriparatide release from optimised NPs led to 64.4% release over 30 days and it showed a diffusion-based mechanism. Based on the favourable results, PHBV/PLGA blend NPs could be a promising candidate for designing a controlled release formulation of Teriparatide.Keywords
Funding Information
- Tehran University of Medical Sciences and Health Services (93-03-33-27074)
This publication has 79 references indexed in Scilit:
- Sustained release of PTH(1–34) from PLGA microspheres suppresses osteoarthritis progression in ratsActa Biomaterialia, 2012
- PHBV/PCL Microparticles for Controlled Release of Resveratrol: Physicochemical Characterization, Antioxidant Potential, and Effect on Hemolysis of Human ErythrocytesThe Scientific World Journal, 2012
- Modeling of drug release from bulk-degrading polymersInternational Journal of Pharmaceutics, 2011
- Optimization of protein encapsulation in PLGA nanoparticlesChemical Engineering and Processing - Process Intensification, 2011
- The Effects of Excipients and Particle Engineering on the Biophysical Stability and Aerosol Performance of Parathyroid Hormone (1-34) Prepared as a Dry Powder for InhalationAAPS PharmSciTech, 2011
- Nanoparticles of Polyethylene Sebacate: A New Biodegradable PolymerAAPS PharmSciTech, 2009
- Box-Behnken design: An alternative for the optimization of analytical methodsAnalytica Chimica Acta, 2007
- Stability and physicochemical characteristics of PLGA, PLGA:poloxamer and PLGA:poloxamine blend nanoparticles: A comparative studyColloids and Surfaces A: Physicochemical and Engineering Aspects, 2007
- Preparation of PLGA nanoparticles containing estrogen by emulsification–diffusion methodColloids and Surfaces A: Physicochemical and Engineering Aspects, 2001
- Some New Three Level Designs for the Study of Quantitative VariablesTechnometrics, 1960