Avian Beta Defensin 2 (AvBD2) Gene Polymorphism Identification in IPB-D1 Chicken
Open Access
- 29 June 2021
- journal article
- research article
- Published by Indonesian Center for Animal Research and Development (ICARD) in Jurnal Ilmu Ternak dan Veteriner
- Vol. 26 (2), 82-88
- https://doi.org/10.14334/jitv.v26i2.2715
Abstract
Avian Beta Defensin 2 (AvBD2) gene, which is located in chromosome 3, plays an important role in the immune system of the chicken by inhibiting the development of microorganisms such as bacteria that infect body tissues. Defensins are produced through epithelial cells immediately after tissue injury or infection, which then processes the maturation of dendritic cells to initiate an immune response in the lymph nodes. The purpose of this study was to discover the polymorphism of the AvBD2 gene in IPB-D1 chickens. PCR and direct-DNA sequencing methods were used to identify the diversity of intron 1, exon 2, and intron 2 AvDB2 genes in 47 chickens. Genotype and allele frequency, and heterozygosity calculations were carried out to obtain information of the AvBD2 gene polymorphism. A total of 10 single nucleotide polymorphisms were found in the AvBD2 gene located in intron 1 (g.4843T>A, g.4853G>A, and g.4859T>C), exon 2 (g.4881A>G, g.4889G>A, and g.5002C>T), and intron 2 (g.5075C>T, g.5111T>G, g.5116G>T, and g.5177G>T). All SNPs are polymorphic. The g.5002C>T mutation causes changes in the amino acid Ala to Val which has the potential to be a candidate for characterizing disease resistance in IPB-D1 chickens.Keywords
This publication has 18 references indexed in Scilit:
- Transition and Transversion Mutations Are Biased towards GC in Transposons of Chilo suppressalis (Lepidoptera: Pyralidae)Genes, 2016
- On the Causes of Evolutionary Transition:Transversion BiasMolecular Biology and Evolution, 2015
- Molecular cloning, characterization and tissue distribution of two ostrich β-defensins: AvBD2 and AvBD7Gene, 2014
- Genome measures used for quality control are dependent on gene function and ancestryBioinformatics, 2014
- Antiviral Mechanisms of Human DefensinsJournal of Molecular Biology, 2013
- Differential gene expression profiles of β-defensins in the crop, intestine, and spleen using a necrotic enteritis model in 2 commercial broiler chicken linesPoultry Science, 2012
- Initial Insights into Structure-Activity Relationships of Avian DefensinsOnline Journal of Public Health Informatics, 2012
- In vitro tests of natural allelic variation of innate immune genes (avian β‐defensins) reveal functional differences in microbial inhibitionJournal of Evolutionary Biology, 2010
- Defensins in innate immunityCell and tissue research, 2010
- A genome-wide screen identifies a single β-defensin gene cluster in the chicken: implications for the origin and evolution of mammalian defensinsBMC Genomics, 2004