Assay for high glucose-mediated islet cell sensitization to apoptosis induced by streptozotocin and cytokines
Open Access
- 1 January 2005
- journal article
- Published by Springer Science and Business Media LLC in Biological Procedures Online
- Vol. 7 (1), 162-171
- https://doi.org/10.1251/bpo113
Abstract
Pancreatic β-cell apoptosis is known to participate in the β-cell destruction process that occurs in diabetes. It has been described that high glucose level induces a hyperfunctional status which could provoke apoptosis. This phenomenon is known as glucotoxicity and has been proposed that it can play a role in type 1 diabetes mellitus pathogenesis. In this study we develop an experimental design to sensitize pancreatic islet cells by high glucose to streptozotocin (STZ) and proinflammatory cytokines [interleukin (IL)-1β, tumor necrosis factor (TNF)-α and interferon (IFN)-γ]-induced apoptosis. This method is appropriate for subsequent quantification of apoptotic islet cells stained with Tdt-mediated dUTP Nick-End Labeling (TUNEL) and protein expression assays by Western Blotting (WB).Keywords
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