Cytotoxicity of the Most Active Fraction of the Seeds of Swietenia macrophylla using Human Breast Cancer MCF-7 Cells

Abstract

Ethyl acetate fraction from Swietenia macrophylla was reported to have toxicity against the larvae of Artemia salina shrimp larvae. However, there areno reports aboutS. macrophylla, which can inhibit human breast cancer cells MCF-7. Therefore, this study aims to evaluate S. macrophyllaextract's cytotoxicity using human breast cancer MCF-7 cells assay, followed by confirmation of its toxicity using brine shrimp lethality assay. The most active fraction obtained from the ethyl acetate extract of S. macrophylla showed 76.49% inhibition at 50 µg/mL (IC₅₀=34.11 µg/mL). At the same time, the most active fraction may contain a mixture of limonoid compounds after LCMS analysis. The most active fraction obtained from ethyl acetate extract of S. macrophylla showed 76.49%