SERS Based Lateral Flow Immunoassay for Point-of-Care Detection of SARS-CoV-2 in Clinical Samples
Top Cited Papers
- 17 March 2021
- journal article
- review article
- Published by American Chemical Society (ACS) in ACS Applied Bio Materials
- Vol. 4 (4), 2974-2995
- https://doi.org/10.1021/acsabm.1c00102
Abstract
The current scenario, an ongoing pandemic of COVID-19, places a dreadful burden on the healthcare system worldwide. Subsequently, there is a need for a rapid, user-friendly, and inexpensive on-site monitoring system for diagnosis. The early and rapid diagnosis of SARS-CoV-2 plays an important role in combating the outbreak. Although conventional methods such as PCR, RT-PCR, and ELISA, etc., offer a gold-standard solution to manage the pandemic, they cannot be implemented as a point-of-care (POC) testing arrangement. Moreover, surface-enhanced Raman spectroscopy (SERS) having a high enhancement factor provides quantitative results with high specificity, sensitivity, and multiplex detection ability but lacks in POC setup. In contrast, POC devices such as lateral flow immunoassay (LFIA) offer rapid, simple-to-use, cost-effective, reliable platform. However, LFIA has limitations in quantitative and sensitive analyses of SARS-CoV-2 detection. To resolve these concerns, herein we discuss a unique modality that is an integration of SERS with LFIA for quantitative analyses of SARS-CoV-2. The miniaturization ability of SERS-based devices makes them promising in biosensor application and has the potential to make a better alternative of conventional diagnostic methods. This review also demonstrates the commercially available and FDA/ICMR approved LFIA kits for on-site diagnosis of SARS-CoV-2.Keywords
Funding Information
- Science and Engineering Research Board (IPA/2020/000130)
This publication has 138 references indexed in Scilit:
- SERS: Materials, applications, and the futureMaterials Today, 2012
- Surface-Enhanced Raman Scattering (SERS) and Surface-Enhanced Resonance Raman Scattering (SERRS): A Review of ApplicationsApplied Spectroscopy, 2011
- A structural analysis of M protein in coronavirus assembly and morphologyJournal of Structural Biology, 2011
- Surface‐enhanced Raman sensors: early history and the development of sensors for quantitative biowarfare agent and glucose detectionJournal of Raman Spectroscopy, 2005
- Development of surface coating technology of TiO2 powder and improvement of photocatalytic activity by surface modificationThin Solid Films, 2005
- Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA DetectionScience, 2002
- Surface-Enhanced Raman Scattering: From Noble to Transition Metals and from Rough Surfaces to Ordered NanostructuresThe Journal of Physical Chemistry B, 2002
- Nanoscale chemical analysis by tip-enhanced Raman spectroscopyChemical Physics Letters, 2000
- Probing Single Molecules and Single Nanoparticles by Surface-Enhanced Raman ScatteringScience, 1997
- Raman spectra of pyridine adsorbed at a silver electrodeChemical Physics Letters, 1974