Objectives: Aim of this study was to determine the Depression, Stress, and Anxiety level among COVID 19 positive healthcare workers working at a tertiary care center of Rohilkhand area during COVID-19 pandemic. Methods: This is a retrospective study was conducted using online survey from March 2020 till Jan 2021 at the Shri Ram Murti Smarak Institute of Medical Sciences Barielly (UP). Participants of the study were fulltime employees of hospital. Data were collected about several aspects of demographic factors, clinical symptoms, co-morbidities and mental health status. We assessed by using an online questionnaire and Depression, Anxiety and Stress - 21 (DASS-21) scale was used. Results: A total of 224 healthcare workers were included who came COVID 19 positive during their duties in SRMS IMS Bareilly, Out of which, 46 (20.5%) participants were suffered from moderate to severe depression, 20.1% (45) from moderate to severe anxiety, and 14.7% (33) from moderate to severe stress. Conclusion: A significant levels of depression, anxiety, and stress were noted with the major concerns of workplace exposure, increased risk of infection, and transmission to their families and friends.