In order to determine the effect of fertilization, environment, and their interactions on the yield and oats yield components. Five fertilization treatments (T1-control, T2-80 kg N ha-1, T3-120 kg N ha-1, 80 kg P2O5 ha-1 and 80 kg K2O ha-1, T4-N120P80K80 + 5 t ha-1 of lime and T5-N120P80K80 + 5 t ha-1 of lime + 30 t ha-1 of farmyard manure) were examined during three growing seasons in Kraljevo location in Western Serbia. Grain yield (GY), 1000 grain weight (TGW), hectolitre weight (HW), plant height (PH), panicle length (PL), number of grains per panicle (NGP) and protein content (PC) were analysed. The aim of the study was to determine the effect of organic and mineral fertilization and calcification on the yield and oats yield components. On average, for all fertilizer variants, during the three-year trial, the highest yield of oats 3802 kg ha-1 was obtained in the fertilizer variant with the combined application of NPK fertilizers, lime and manure. Positive highly significant correlation, during in the study trial were found between yields with PH, PL and NGP. Negative and highly significant dependencies were found between protein content with GY, PH and NGP. The results of these studies indicate the importance of rational introduction of adequate quantities of fertilization, calcification and humization in order to make oats as profitable as possible in the agro-ecological conditions in Pannonian Environments.