Listeria monocytogenes is a food-borne intracellular pathogen that is resistant to adverse conditions of food processing and is a causative agent of high mortality listeriosis disease. Analysis of large food lots is limited by culture methods, which are considered the gold standard. Nowadays, the researches of sensitive, fast and reliable method are focused on. Biosensors with on-site detection potential and their characterizing with high accuracy and precision offers many opportunities for real-time estimation of industrial contamination. As the most promising technique for rapid detection of Listeria monocytogenes, ones based on surface plasmon resonance (SPR) and fiber optic biosensors (FOBs) where direct or indirect detections can be made by detecting the interaction of the biomarker element with the optical field without marking or labeling, have come to the fore. SPR and FOBs can be detected directly in the range of 102-106 cfu/ml and ≤101-103 cfu/ml, respectively.