Currently, the pharmaceutical industry is constantly working on the creation of ideal disinfectants that would have a wide spectrum of antimicrobial action in minimal concentrations, did not result in the formation of stability in bacteria, were not toxic, not corrosive, not allergenic, cheap. The purpose of the work was to investigate the activity of the “Enzidesis” disidissance to influence microbial biofilms in vitro conditions for various sampling parameters. The density of microbial biofilms and the effects of disinfectants were determined on sterile stainless steel plates, which were placed in a Petri dish. For this purpose, biofilms were grown on plates, washed out biofilms from planktonic cells, acted as a disguise for a certain concentration and for a period of 5–30 minutes, fixed and painted biofilms and determined the optical density of the flushing solution from biofilms spectrometrically. It has been established that the disinfectant “Enzidesis” destroys the biofilms taken in the experiment of museum test cultures S. aureus, E. coli and P. aeruginosa. In particular, for the influence of the lowest concentration in experiment, 0.075 % optical density of washing solutions from S. aureus biofilmes decreased by 2.6 times, E. coli and P. aeruginosa biofilm in 2.9 times, respectively, comparing with biofilms after treatment with water. Under the actions of such a concentration of the “Enzidesis” means, the biofilm though significantly degraded, but they were still a medium density, more than 0.5 units. Increasing the concentration of a means of 0.075 % to 0.5 % contributed to the intensity of degradation of test-cultures, an average of 3.0 times (P < 0.05) and they became a weak density (0.24–0.20 s). Increasing the concentration of the “Enzidesis” means up to 1.0 % or more, did not significantly destroy the matrix of the biofilm of microorganisms, since the optical density of the washing solutions was as in control. In determining the influence of the temperature of the working solutions of the “Enzidosis” means on its film-building activity, it has been found that with an increase in the disinfection agent “Enzidesis” with +20 to +60 °С there is an increase in the degradation of the biofilm formed S. aureus, E. coli and P. aeruginosa. Dezilities can be effectively used in 0.5 % concentration at room temperature of solutions. When substantiating the exposure time of the “Enzidesis” exhibition time, it has been found that to remove the S. aureus, E. coli and P. aeruginosa 0.5 % means at a solution temperature of +20 ± 1 °C, it is necessary that the action time is from 15 to 30 minutes.